Human NCS1 Recombinant Protein (RPPB4070)

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SKU:
RPPB4070
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P62166

Description

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Product Name:Human NCS1 Recombinant Protein
Product Code:RPPB4070
Size:25µg
Species:Human
Target:NCS1
Synonyms:Frequenin homolog, FLUP, FREQ, Neuronal calcium sensor 1, Frequenin-like protein, Frequenin-like ubiquitous protein, NCS-1, NCS1, DKFZp761L1223.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear colorless solution.
Formulation:The NCS1 solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH

NCS1 is part of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed primarily in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. NCS1 is related with secretory granules and modulates synaptic transmission and synaptic plasticity. NCS1 regulates GRK1 and substitutes for calmodulin. NCS1 stimulates PI4KB kinase activity and participates in long-term synaptic plasticity through its interaction with PICK1. NCS1 takes part in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

NCS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 198 amino acids (1-190 a.a.) and having a molecular mass of 22.9 kDa. The NCS1 is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:FREQ: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with IL1RAPL1. Belongs to the recoverin family.
UniProt Protein Details:

Protein type:Calcium-binding

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoplasm; intracellular membrane-bound organelle; plasma membrane

Molecular Function:calcium ion binding; protein binding; voltage-gated calcium channel activity

NCBI Summary:This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P62166
NCBI GenInfo Identifier:49065666
NCBI Gene ID:23413
NCBI Accession:P62166.2
UniProt Secondary Accession:P62166,P36610, Q9UK26, E9PAY3,
UniProt Related Accession:P62166
Molecular Weight:19,728 Da
NCBI Full Name:Neuronal calcium sensor 1
NCBI Synonym Full Names:neuronal calcium sensor 1
NCBI Official Symbol:NCS1��
NCBI Official Synonym Symbols:FLUP; FREQ��
NCBI Protein Information:neuronal calcium sensor 1
UniProt Protein Name:Neuronal calcium sensor 1
UniProt Synonym Protein Names:Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein
Protein Family:Frequenin
UniProt Gene Name:NCS1��
UniProt Entry Name:NCS1_HUMAN
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