Human NCK1 Recombinant Protein (RPPB4065)
- SKU:
- RPPB4065
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P16333
Description
| Product Name: | Human NCK1 Recombinant Protein |
| Product Code: | RPPB4065 |
| Size: | 25µg |
| Species: | Human |
| Target: | NCK1 |
| Synonyms: | NCK, NCKalpha, NCK1, NCK adaptor protein 1, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha, MGC12668. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | NCK1 Human solution containing 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA, 50mM NaCl & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS |
NCK1 is an adapter protein which binds with tyrosine-phosphorylated growth factor receptors or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 is involved in the DNA damage response, for efficient activation of downstream effectors, such as that of CHEK2. NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. NCK1 is located in the cytoplasm and is an adaptor protein that participates in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.
NCK1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 397 amino acids (1-377 a.a.) and having a molecular mass of 45 kDa. The NCK1 is fused to�a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NCK1: adapter protein containing Src homology 2 and 3 (SH2 and SH3) domains. Transduces signals from tyrosine-phosphorylated growth factor receptors to their cellular substrates. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q21 Cellular Component: endoplasmic reticulum; cytoplasm; plasma membrane; ribosome; protein phosphatase type 1 complex; intercellular junction; vesicle membrane; cytosol; nucleus Molecular Function:protein binding, bridging; protein domain specific binding; protein binding; receptor signaling complex scaffold activity; protein kinase inhibitor activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity; receptor tyrosine kinase binding; receptor binding Biological Process: lamellipodium biogenesis; axon guidance; positive regulation of translation in response to stress; T cell activation; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; response to other organism; T cell receptor signaling pathway; substrate-bound cell migration, cell extension; positive regulation of actin filament polymerization; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P16333 |
| NCBI GenInfo Identifier: | 127962 |
| NCBI Gene ID: | 4690 |
| NCBI Accession: | P16333.1 |
| UniProt Secondary Accession: | P16333,B7Z751, D3DNE3, |
| UniProt Related Accession: | P16333 |
| Molecular Weight: | 377 |
| NCBI Full Name: | Cytoplasmic protein NCK1 |
| NCBI Synonym Full Names: | NCK adaptor protein 1 |
| NCBI Official Symbol: | NCK1�� |
| NCBI Official Synonym Symbols: | NCK; nck-1; NCKalpha�� |
| NCBI Protein Information: | cytoplasmic protein NCK1; NCK tyrosine kinase; melanoma NCK protein; SH2/SH3 adaptor protein NCK-alpha; non-catalytic region of tyrosine kinase |
| UniProt Protein Name: | Cytoplasmic protein NCK1 |
| UniProt Synonym Protein Names: | NCK adaptor protein 1; Nck-1; SH2/SH3 adaptor protein NCK-alpha |
| Protein Family: | Nck-associated protein |
| UniProt Gene Name: | NCK1�� |
| UniProt Entry Name: | NCK1_HUMAN |
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