Human NCF1 Recombinant Protein (RPPB5908)
- SKU:
- RPPB5908
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P14598
Description
| Product Name: | Human NCF1 Recombinant Protein |
| Product Code: | RPPB5908 |
| Size: | 10µg |
| Species: | Human |
| Target: | NCF1 |
| Synonyms: | NCF1A, NOXO2, p47phox, SH3PXD1A . |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 0.5mg/ml solution containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCST LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG EAVEVIHKLL DGWWVIRKDDVTGYFPSMYL QKSGQDVSQA QRQIKRGAPP RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV VEHHHHHH |
NCF1 is a cytosolic subunit protein of neutrophil NADPH oxidase which aMulti-component enzyme that is activates production of superoxide anion. NCF1, along with NCF2 and a membrane bound cytochrome b558, is necessary for activation of the latent NADPH oxidase necessary for superoxide production. Mutations in this NCF1 have been related with chronic granulomatous disease.
NCF1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-390 a.a.) and having a molecular mass of 45.7 kDa. The NCF1 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.-.-.- Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex Molecular Function:electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell proliferation; cell redox homeostasis; cellular defense response; hydrogen peroxide biosynthetic process; inflammatory response; innate immune response; leukotriene metabolic process; negative regulation of smooth muscle contraction; protein targeting to membrane; respiratory burst; respiratory burst during defense response; response to yeast; small GTPase mediated signal transduction; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I |
| NCBI Summary: | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14598 |
| NCBI GenInfo Identifier: | 325511390 |
| NCBI Gene ID: | 653361 |
| NCBI Accession: | P14598.3 |
| UniProt Secondary Accession: | P14598,O43842, Q2PP07, Q53FR5, Q9BU90, Q9BXI7, Q9BXI8 Q9UDV9, Q9UMU2, A6NEH2, A8K7S9, |
| UniProt Related Accession: | P14598 |
| Molecular Weight: | |
| NCBI Full Name: | Neutrophil cytosol factor 1 |
| NCBI Synonym Full Names: | neutrophil cytosolic factor 1 |
| NCBI Official Symbol: | NCF1�� |
| NCBI Official Synonym Symbols: | NCF1A; NOXO2; p47phox; SH3PXD1A�� |
| NCBI Protein Information: | neutrophil cytosol factor 1 |
| UniProt Protein Name: | Neutrophil cytosol factor 1 |
| UniProt Synonym Protein Names: | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox |
| Protein Family: | Neutrophil cytosol factor |
| UniProt Gene Name: | NCF1�� |
| UniProt Entry Name: | NCF1_HUMAN |
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