Enzymes Recombinant Proteins

Human NAGK Recombinant Protein (RPPB2587)

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SKU:
RPPB2587
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UJ70
Research Area:
Enzymes

Description

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Product Name:Human NAGK Recombinant Protein
Product Code:RPPB2587
Size:20µg
Species:Human
Target:NAGK
Synonyms:N-acetylglucosamine kinase, GNK, GlcNAc kinase, N-acetyl-D-glucosamine kinase, HSA242910, EC 2.7.1.59.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NAGK solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 2mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS

NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate. NAGK is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK cooperates with STK16 and LNX1 and has ManNAc kinase activity.

NAGK Human Recombinant produced in E. coli is a single polypeptide chain containing 367 amino acids (1-344) and having a molecular mass of 39.8kDa.NAGK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NAGK: Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity. Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
UniProt Protein Details:

Protein type:EC 2.7.1.59; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 2p13.3

Molecular Function:protein binding; N-acetylglucosamine kinase activity; ATP binding; N-acylmannosamine kinase activity

Biological Process: N-acetylneuraminate catabolic process; N-acetylglucosamine metabolic process; carbohydrate phosphorylation; N-acetylmannosamine metabolic process

NCBI Summary:This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars. [provided by RefSeq, Nov 2011]
UniProt Code:Q9UJ70
NCBI GenInfo Identifier:48146659
NCBI Gene ID:55577
NCBI Accession:CAG33552.1
UniProt Secondary Accession:Q9UJ70,Q53HD5, Q6IA84, Q9BS29, Q9BVP0, Q9NV37, B4DLZ5
UniProt Related Accession:Q9UJ70
Molecular Weight:37,376 Da
NCBI Full Name:NAGK
NCBI Synonym Full Names:N-acetylglucosamine kinase
NCBI Official Symbol:NAGK��
NCBI Official Synonym Symbols:GNK; HSA242910��
NCBI Protein Information:N-acetyl-D-glucosamine kinase; glcNAc kinase
UniProt Protein Name:N-acetyl-D-glucosamine kinase
UniProt Synonym Protein Names:GlcNAc kinase
Protein Family:N-acetyl-D-glucosamine kinase
UniProt Gene Name:NAGK��
UniProt Entry Name:NAGK_HUMAN
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