Human MYL5 (1-173 a.a.) Recombinant Protein (RPPB4036)

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RPPB4036

Description

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Product Name:Human MYL5 (1-173 a.a.) Recombinant Protein
Product Code:RPPB4036
Size:10µg
Species:Human
Target:MYL5 (1-173 a.a.)
Synonyms:Myosin light chain 5, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2, MYLC2, MyLC-2, MYL5.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:MYL5 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl,1mM DTT and 0.1mM PMSF.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNLDYKALSYVIT HGEEKEE

Myosin regulatory light chain 5 (MYL5) is a hexameric ATPase cellular motor protein. Myosin is comprised of 2 heavy chains, 2 nonphosphorylatable alkali light chains, and 2 phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in the fetal muscle and in the adult retina, cerebellum, and basal ganglia. The reconstitution of myosin with MYL5 or alkali light chain increases filament velocity to intermediate rates, and the re-addition of both classes of light chains fully reinstates the original sliding pace.

MYL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (1-173 a.a.) and having a molecular mass of 22.1kDa.MYL5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MYL5: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxis; ContractileChromosomal Location of Human Ortholog: 4p16.3Cellular Component: muscle myosin complexMolecular Function: structural constituent of muscle; calcium ion bindingBiological Process: regulation of muscle contraction
UniProt Protein Details:
NCBI Summary:This gene encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene product, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. [provided by RefSeq, Jul 2008]
UniProt Code:Q02045
NCBI GenInfo Identifier:4505305
NCBI Gene ID:4636
NCBI Accession:NP_002468.1
UniProt Secondary Accession:Q02045,Q8IXL8
UniProt Related Accession:Q02045
Molecular Weight:19,534 Da
NCBI Full Name:myosin light chain 5
NCBI Synonym Full Names:myosin, light chain 5, regulatory
NCBI Official Symbol:MYL5��
NCBI Official Synonym Symbols:
NCBI Protein Information:myosin light chain 5; MYLC2; myLC-2; myosin regulatory light chain 5; myosin, light polypeptide 5, regulatory; superfast myosin regulatory light chain 2
UniProt Protein Name:Myosin light chain 5
UniProt Synonym Protein Names:Myosin regulatory light chain 5; Superfast myosin regulatory light chain 2; MYLC2; MyLC-2
Protein Family:Myosin
UniProt Gene Name:MYL5��
UniProt Entry Name:MYL5_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q02045
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