Human MYL12B Recombinant Protein (RPPB4032)

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SKU:
RPPB4032
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O14950

Description

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Product Name:Human MYL12B Recombinant Protein
Product Code:RPPB4032
Size:20µg
Species:Human
Target:MYL12B
Synonyms:Myosin light chain 12B regulatory, Myosin regulatory light chain 2-B smooth muscle isoform, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, MYLC2B, MRLC2, MLC-2A, MLC-B, SHUJUN-1 MLC20.�
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The MYL12B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM Nacl, 2mM DTT and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD

Myosin is composed of 2 nonphosphorylatable alkali light chains, 2 heavy chains and 2 phosphorylatable regulatory light chains. MYL12B is a hexameric ATPase cellular motor protein which controls contraction in smooth muscle and non-muscle cells through phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains catalyzed by MLCK in the presence of calcium and calmodulin, rises Actin-activated myosin ATPase activity, thus regulating contractile activity.

MYL12B Human Recombinant produced in�E. coli is a single polypeptide chain containing 196 amino acids (1-172) and having a molecular mass of 22.3kDa.MYL12B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MRLC2: non-sarcomeric myosin regulatory light chain 2. Binds calcium and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Phosphorylation of MLC-2 in the presence of calcium and calmodulin increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. Myosin is an hexamer of 2 heavy chains and 4 light chains.
UniProt Protein Details:

Protein type:Contractile

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: cytosol

Molecular Function:protein binding

Biological Process: muscle contraction

NCBI Summary:This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]
UniProt Code:O14950
NCBI GenInfo Identifier:205829213
NCBI Gene ID:10627
NCBI Accession:O14950.2
UniProt Secondary Accession:O14950,Q13182, Q7Z5Z4, D3DUH6,
UniProt Related Accession:P19105
Molecular Weight:19,779 Da
NCBI Full Name:Myosin regulatory light chain 12B
NCBI Synonym Full Names:myosin light chain 12A
NCBI Official Symbol:MYL12A��
NCBI Official Synonym Symbols:MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24��
NCBI Protein Information:myosin regulatory light chain 12A
UniProt Protein Name:Myosin regulatory light chain 12B
UniProt Synonym Protein Names:MLC-2A; MLC-2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2; SHUJUN-1
Protein Family:Myosin regulatory
UniProt Gene Name:MYL12B��
UniProt Entry Name:ML12B_HUMAN
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