Enzymes Recombinant Proteins

Human MTHFS Recombinant Protein (RPPB1984)

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RPPB1984

Description

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Product Name:Human MTHFS Recombinant Protein
Product Code:RPPB1984
Size:25µg
Species:Human
Target:MTHFS
Synonyms:5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase), HsT19268, Methenyl-THF synthetase, FLJ30410, EC 6.3.3.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The MTHFS protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 5mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA

MTHFS is a cytosolic protein which takes part in the formate metabolic process. MTHFS along with a magnesium cofactor catalyzes the ATP-dependent reaction which reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate(MTHF). MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. In addition, MTHF is a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD).

MTHFS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-203a.a.) and having a molecular mass of 25.4 kDa. MTHFS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.
NCBI Summary:The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
UniProt Code:P49914
NCBI GenInfo Identifier:1706921
NCBI Gene ID:10588
NCBI Accession:P49914.2
UniProt Related Accession:P49914
Molecular Weight:20kDa; 23kDa
NCBI Full Name:5-formyltetrahydrofolate cyclo-ligase
NCBI Synonym Full Names:methenyltetrahydrofolate synthetase
NCBI Official Symbol:MTHFS��
NCBI Official Synonym Symbols:NEDMEHM; HsT19268��
NCBI Protein Information:5-formyltetrahydrofolate cyclo-ligase
UniProt Protein Name:5-formyltetrahydrofolate cyclo-ligase
UniProt Synonym Protein Names:5,10-methenyl-tetrahydrofolate synthetase; MTHFS; Methenyl-THF synthetase
UniProt Gene Name:MTHFS��
UniProt Entry Name:MTHFS_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49914
Research Area:
Enzymes
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