Human MRRF Recombinant Protein (RPPB3999)
- SKU:
- RPPB3999
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96E11
Description
| Product Name: | Human MRRF Recombinant Protein |
| Product Code: | RPPB3999 |
| Size: | 20µg |
| Species: | Human |
| Target: | MRRF |
| Synonyms: | MRFF, MTRRF, RRF, Ribosome-recycling factor, mitochondrial, Ribosome-releasing factor, mitochondrial. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | MRRF protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer, (pH 8.0), 0.2M NaCl, 30% glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG |
Mitochondrial Ribosome Recycling Factor (MRRF) is a member of the RRF family. MRRF attaches to the large ribosomal subunit in the cleft which has a peptidyl transferase center. MRRF controls the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may intensify the efficacy of translation by recycling ribosome from one round of translation to another.
MRRF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (56-262 a.a.) and having a molecular mass of 25.1kDa.MRRF is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MRRF: Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. Belongs to the RRF family. 8 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 9q33.2Cellular Component: mitochondrial matrix; mitochondrionMolecular Function: ribosomal large subunit bindingBiological Process: ribosome disassembly |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q96E11 |
| NCBI GenInfo Identifier: | 62901043 |
| NCBI Gene ID: | 92399 |
| NCBI Accession: | Q96E11.1 |
| UniProt Secondary Accession: | Q96E11,Q5RKT1, Q5T7T0, Q5T7T1, Q5T7T2, Q5T7T3, Q5T7T4 Q5T7T5, A8K6D8, A8K6Z4 |
| UniProt Related Accession: | Q96E11 |
| Molecular Weight: | 23,511 Da |
| NCBI Full Name: | Ribosome-recycling factor, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosome recycling factor |
| NCBI Official Symbol: | MRRF�� |
| NCBI Official Synonym Symbols: | RRF; MRFF; MTRRF�� |
| NCBI Protein Information: | ribosome-recycling factor, mitochondrial |
| UniProt Protein Name: | Ribosome-recycling factor, mitochondrial |
| UniProt Synonym Protein Names: | Ribosome-releasing factor, mitochondrial |
| Protein Family: | |
| UniProt Gene Name: | MRRF�� |
| UniProt Entry Name: | RRFM_HUMAN |
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