Human MRPS25 Recombinant Protein (RPPB4008)

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SKU:
RPPB4008
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P82663

Description

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Product Name:Human MRPS25 Recombinant Protein
Product Code:RPPB4008
Size:20µg
Species:Human
Target:MRPS25
Synonyms:Mitochondrial Ribosomal Protein S25, Mitochondrial 28S Ribosomal Protein S25, 28S Ribosomal Protein S25 Mitochondrial, MRP-S25, RPMS25, S25mt.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The MRPS25 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMPMKGRF PIRRTLQYLS QGNVVFKDSV KVMTVNYNTH GELGEGARKF VFFNIPQIQY KNPWVQIMMF KNMTPSPFLR FYLDSGEQVL VDVETKSNKE IMEHIRKILG KNEETLREEE EEKKQLSHPA NFGPRKYCLR ECICEVEGQV PCPSLVPLPK EMRGKYKAAL KADAQD

MRPS25, a 28S subunit protein, is a member of the ribosomal protein S5P family. An equivalent pseudogene is located on chromosome 4.

MRPS25 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-173) and having a molecular mass of 22.5kDa.MRPS25 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MRPS25: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: mitochondrial inner membrane; mitochondrion; ribosome

Molecular Function:structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:P82663
NCBI GenInfo Identifier:13633894
NCBI Gene ID:64432
NCBI Accession:P82663.1
UniProt Secondary Accession:P82663,Q9H7P5, B4DFJ5, B4DQG6,
UniProt Related Accession:P82663
Molecular Weight:14,953 Da
NCBI Full Name:28S ribosomal protein S25, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein S25
NCBI Official Symbol:MRPS25��
NCBI Official Synonym Symbols:RPMS25; MRP-S25��
NCBI Protein Information:28S ribosomal protein S25, mitochondrial
UniProt Protein Name:28S ribosomal protein S25, mitochondrial
Protein Family:28S ribosomal protein
UniProt Gene Name:MRPS25��
UniProt Entry Name:RT25_HUMAN
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