Human MRPL28 Recombinant Protein (RPPB4003)

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SKU:
RPPB4003
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13084

Description

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Product Name:Human MRPL28 Recombinant Protein
Product Code:RPPB4003
Size:10µg
Species:Human
Target:MRPL28
Synonyms:MAAT1, p15, 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma-associated antigen recognized by T-lymphocytes.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The MRPL28 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.5), 0.1M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ

MRPL28 is found in a variety of normal tissues such as spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. 39S ribosomal protein L28, mitochondrial (MRPL28), is a part of the ribosomal protein L28P family. Mammalian mitochondrial ribosomal proteins encoded by nuclear genes and helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL28 is an important therapeutic reagent for HLA-A24 (A24) patients since this antigen is distinguished by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype.

MRPL28 Human Recombinant produced in E. coli is a single polypeptide chain containing 222 amino acids (56-256) and having a molecular mass of 25.8 kDa. MRPL28 is fused to 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MRPL28: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Ribosomal; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function:protein binding; structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]
UniProt Code:Q13084
NCBI GenInfo Identifier:85695426
NCBI Gene ID:10573
NCBI Accession:Q13084.4
UniProt Secondary Accession:Q13084,Q4TT39, Q96S26, Q9BQD8, Q9BR04, B2RCM4, D3DU46
UniProt Related Accession:Q13084
Molecular Weight:256
NCBI Full Name:39S ribosomal protein L28, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L28
NCBI Official Symbol:MRPL28��
NCBI Official Synonym Symbols:p15; MAAT1��
NCBI Protein Information:39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; melanoma antigen p15; melanoma-associated antigen recognized by T lymphocytes; melanoma-associated antigen recognized by T-lymphocytes; melanoma-associated antigen recognised by cytotoxic T lymphocytes
UniProt Protein Name:39S ribosomal protein L28, mitochondrial
UniProt Synonym Protein Names:Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes
Protein Family:39S ribosomal protein
UniProt Gene Name:MRPL28��
UniProt Entry Name:RM28_HUMAN
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