Human MRPL2 Recombinant Protein (RPPB4002)

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SKU:
RPPB4002
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q5T653

Description

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Product Name:Human MRPL2 Recombinant Protein
Product Code:RPPB4002
Size:20µg
Species:Human
Target:MRPL2
Synonyms:39S ribosomal protein L2, mitochondrial , CGI-22,MRP-L14, RPML14, L2mt, MRP-L2, CGI-22.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:MRPL2 protein solution (0.25mg/ml) containing 20mMPhosphate buffer (pH 8.0), 1mM EDTA, 50% glycerol and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVRYDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GR

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and aid in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) comprised of a small 28S subunit and a large 39S subunit. Among different species, the proteins comprising the mitoribosome vary greatly in sequence, and sometimes in biochemical properties, thus preventing simple recognition by sequence homology. Mitochondrial Ribosomal Protein L2 (MRPL2) is a 39S subunit protein which is a member of the EcoL2 ribosomal protein family.

MRPL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (84-202 a.a) and having a molecular mass of 15.5kDa.MRPL2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MRPL2: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Ribosomal; Mitochondrial; RNA-binding; Motility/polarity/chemotaxis; Translation

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function:structural constituent of ribosome

Biological Process: mitochondrial translation

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:Q5T653
NCBI GenInfo Identifier:118573672
NCBI Gene ID:51069
NCBI Accession:Q5T653.2
UniProt Secondary Accession:Q5T653,Q8WUL1, Q96Q56, Q9Y311, B2RC56,
UniProt Related Accession:Q5T653
Molecular Weight:33,301 Da
NCBI Full Name:39S ribosomal protein L2, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L2
NCBI Official Symbol:MRPL2��
NCBI Official Synonym Symbols:CGI-22; RPML14; MRP-L14��
NCBI Protein Information:39S ribosomal protein L2, mitochondrial
UniProt Protein Name:39S ribosomal protein L2, mitochondrial
Protein Family:39S ribosomal protein
UniProt Gene Name:MRPL2��
UniProt Entry Name:RM02_HUMAN
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