Human MRPL13 Recombinant Protein (RPPB4004)

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SKU:
RPPB4004
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BYD1

Description

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Product Name:Human MRPL13 Recombinant Protein
Product Code:RPPB4004
Size:20µg
Species:Human
Target:MRPL13
Synonyms:L13, L13A, L13mt, RPL13, RPML13, 39S ribosomal protein L13, mitochondrial, MRPL13.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:MRPL13 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSSFSRA PQQWATFARI WYLLDGKMQP PGKLAAMASI RLQGLHKPVY HALSDCGDHV VIMNTRHIAF SGNKWEQKVY SSHTGYPGGF RQVTAAQLHL RDPVAIVKLA IYGMLPKNLH RRTMMERLHL FPDEYIPEDI LKNLVEELPQ PRKIPKRLDE YTQEEIDAFP RLWTPPEDYR L

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and assist in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit (MRPL13 is a 39S subunit protein) and have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. An additional difference between mammalian mitoribosomes and prokaryotic ribosomes is that the second contain a 5S rRNA. Among various species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.

MRPL13 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-178 a.a.) and having a molecular mass of 23.1kDa. MRPL13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MRPL13: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Translation; RNA-binding; Mitochondrial; Ribosomal

Chromosomal Location of Human Ortholog: 8q22.1-q22.3

Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrial large ribosomal subunit

Molecular Function:protein binding; structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BYD1
NCBI GenInfo Identifier:22257023
NCBI Gene ID:28998
NCBI Accession:Q9BYD1.1
UniProt Secondary Accession:Q9BYD1,Q9UI04, B2R4R8,
UniProt Related Accession:Q9BYD1
Molecular Weight:178
NCBI Full Name:39S ribosomal protein L13, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L13
NCBI Official Symbol:MRPL13��
NCBI Official Synonym Symbols:L13; L13A; L13mt; RPL13; RPML13��
NCBI Protein Information:39S ribosomal protein L13, mitochondrial; MRP-L13
UniProt Protein Name:39S ribosomal protein L13, mitochondrial
Protein Family:39S ribosomal protein
UniProt Gene Name:MRPL13��
UniProt Entry Name:RM13_HUMAN
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