Enzymes Recombinant Proteins
Human MPST Recombinant Protein (RPPB5820)
- SKU:
- RPPB5820
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P25325
- Research Area:
- Enzymes
Description
| Product Name: | Human MPST Recombinant Protein |
| Product Code: | RPPB5820 |
| Size: | 20µg |
| Species: | Human |
| Target: | MPST |
| Synonyms: | 3-mercaptopyruvate sulfurtransferase, MST, MPST, TST2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The MPST solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMASPQL CRALVSAQWV AEALRAPRAG QPLQLLDASW YLPKLGRDAR REFEERHIPG AAFFDIDQCS DRTSPYDHML PGAEHFAEYA GRLGVGAATH VVIYDASDQG LYSAPRVWWM FRAFGHHAVS LLDGGLRHWL RQNLPLSSGK SQPAPAEFRA QLDPAFIKTY EDIKENLESR RFQVVDSRAT GRFRGTEPEP RDGIEPGHIP GTVNIPFTDF LSQEGLEKSP EEIRHLFQEK KVDLSKPLVA TCGSGVTACH VALGAYLCGK PDVPIYDGSW VEWYMRARPE DVISEGRGKT H |
Mercaptopyruvate Sulfurtransferase (MPST) catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. MPST might be involved in cysteine degradation and cyanide detoxification. The MPST enzyme is regulated by oxidative stress and thioredoxin. In oxidative stress conditions, the catalytic cysteine site is transformed to a sulfenate which inhibits the MPST enzyme activity. The reduced thioredoxin cleaves an intersubunit disulfide bond to activate the redox switch and reactivate the enzyme. A deficiency in MPST activity is implicated in a rare inheritable condition known as MCDU (mercaptolactate-cysteine disulfiduria).
MPST Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-297) and having a molecular mass of 35kDa.MPST is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MPST: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - cysteine and methionine; EC 2.8.1.2; Mitochondrial; Transferase Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: neuron projection Biological Process: cyanate catabolic process; response to toxin |
| NCBI Summary: | This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25325 |
| NCBI GenInfo Identifier: | 6226903 |
| NCBI Gene ID: | 4357 |
| NCBI Accession: | P25325.3 |
| UniProt Secondary Accession: | P25325,O75750, Q6FHN9, A8MZ34, B3KP52, J3KPV7, |
| UniProt Related Accession: | P25325 |
| Molecular Weight: | 33kDa |
| NCBI Full Name: | 3-mercaptopyruvate sulfurtransferase |
| NCBI Synonym Full Names: | mercaptopyruvate sulfurtransferase |
| NCBI Official Symbol: | MPST�� |
| NCBI Official Synonym Symbols: | MST; TST2; TUM1�� |
| NCBI Protein Information: | 3-mercaptopyruvate sulfurtransferase |
| UniProt Protein Name: | 3-mercaptopyruvate sulfurtransferase |
| Protein Family: | 3-mercaptopyruvate sulfurtransferase |
| UniProt Gene Name: | MPST�� |
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