Enzymes Recombinant Proteins

Human MPO Recombinant Protein (RPPB2075)

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RPPB2075

Description

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Product Name:Human MPO Recombinant Protein
Product Code:RPPB2075
Size:25µg
Species:Human
Target:MPO
Synonyms:Myeloperoxidase, EC 1.11.1.7, MPO.
Source:Human peripheral blood polymorphonuclear leukocytes
Physical Appearance:Sterile filtered colorless solution.
Formulation:MPO solution is supplied in 20mM HEPES buffer pH-7.5, 200mM NaCl and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.

Myeloperoxidase is an important enzyme used by granulocytes during phagocytic lysis of foreign particles engulfed. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas. MPO is useful in differentiating between myeloid and lymphoid leukemias.

MPO is a natural protein having a molecular mass of 150kDa containing 2 subunits each of a heavy chain with 64kDa and a light chain with 13kDa. MPO is isolated from human peripheral blood polymorphonuclear leukocytes.

UniProt Protein Function:MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function:heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; defense response; response to yeast; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease

NCBI Summary:Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
UniProt Code:P05164
NCBI GenInfo Identifier:129825
NCBI Gene ID:4353
NCBI Accession:P05164.1
UniProt Secondary Accession:P05164,Q14862, Q4PJH5, Q9UCL7, A1L4B8,
UniProt Related Accession:P05164
Molecular Weight:87,248 Da
NCBI Full Name:Myeloperoxidase
NCBI Synonym Full Names:myeloperoxidase
NCBI Official Symbol:MPO��
NCBI Protein Information:myeloperoxidase
UniProt Protein Name:Myeloperoxidase
Protein Family:Myeloperoxidase
UniProt Gene Name:MPO��
UniProt Entry Name:PERM_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P05164
Research Area:
Enzymes
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