Enzymes Recombinant Proteins

Human MPG Recombinant Protein (RPPB1975)

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SKU:
RPPB1975
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P29372
Research Area:
Enzymes

Description

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Product Name:Human MPG Recombinant Protein
Product Code:RPPB1975
Size:10µg
Species:Human
Target:MPG
Synonyms:DNA-3-methyladenine glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, AAG, ANPG, MID1, MDG, PIG11, PIG16, CRA36.1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:MPG protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol, 200mM NaCl and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQALE HHHHHH

DNA-3-methyladenine glycosylase (MPG) is a member of the DNA glycosylase MPG family. MPG initiates base excision repair in DNA by removing a wide variety of alkylated, deaminated, and lipid peroxidation-induced purine adducts.

MPG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids (1-298 a.a.) and having a molecular mass of 33.9kDa (Molecular weight on SDS-PAGE will appear higher).MPG is fused to an 8 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.2.2.21; Hydrolase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm

Molecular Function:protein binding; alkylbase DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; damaged DNA binding

Biological Process: DNA dealkylation; base-excision repair, AP site formation; depurination; base-excision repair; DNA repair

UniProt Code:P29372
NCBI GenInfo Identifier:206729922
NCBI Gene ID:4350
NCBI Accession:P29372.3
UniProt Secondary Accession:P29372,Q13770, Q15275, Q15961, Q5J9I4, Q96BZ6, Q96S33 Q9NNX5, G5E9E2,
UniProt Related Accession:P29372
Molecular Weight:298
NCBI Full Name:DNA-3-methyladenine glycosylase
NCBI Synonym Full Names:N-methylpurine-DNA glycosylase
NCBI Official Symbol:MPG��
NCBI Official Synonym Symbols:AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1��
NCBI Protein Information:DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; proliferation-inducing protein 11; proliferation-inducing protein 16; N-methylpurine-DNA glycosylase, MPG; CRA36.1 (3-methyl-adenine DNA glycosylase); 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p
UniProt Protein Name:DNA-3-methyladenine glycosylase
UniProt Synonym Protein Names:3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase
Protein Family:DNA-3-methyladenine glycosylase
UniProt Gene Name:MPG��
UniProt Entry Name:3MG_HUMAN
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