Human/Mouse/Rat Phosphotyrosine STAT5 PharmaGenie ELISA Kit (SBRS1992)

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SKU:
SBRS1992
Product Type:
ELISA Kit
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human/Mouse/Rat Phosphotyrosine STAT5 PharmaGenie ELISA Kit (SBRS1992)

Assay Genie Phospho-Stat5 ELISA (Enzyme-Linked Immuno-sorbent Assay) kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phospho-Stat5 in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and Rat phospho-Stat5. An anti-Stat5 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Stat5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Stat5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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