Human/Mouse/Rat Phospho-SHP2 (Y542) and Total SHP2 PharmaGenie ELISA Kit (SBRS1967)

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SKU:
SBRS1967
Product Type:
ELISA Kit
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human/Mouse/Rat Phospho-SHP2 (Y542) and Total SHP2 PharmaGenie ELISA Kit (SBRS1967)

Assay Genie Phospho- SHP2 (Y542) and Total SHP2 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated SHP2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-SHP2 and total SHP2. An anti-pan SHP2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SHP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-SHP2 (Y542) antibody is used to detect phosphorylated SHP2 or biotinylated anti-SHP2 antibody is used to detect pan SHP2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SHP2 (Y542) or pan SHP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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