Human/Mouse/Rat Phospho-P53 (S15) and Total P53 PharmaGenie ELISA Kit (SBRS1918)

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SKU:
SBRS1918
Product Type:
ELISA Kit
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human/Mouse/Rat Phospho-P53 (S15) and Total P53 PharmaGenie ELISA Kit (SBRS1918)

Assay Genie Phospho-P53 (S15) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated P53 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-P53. An anti-P53 (S15) antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated P53 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-P53 antibody is used to detect phosphorylated P53. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P53 (S15). The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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