Human/Mouse/Rat Phospho-MKK3 (S189) and Total MKK3 PharmaGenie ELISA Kit (SBRS1897)

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SKU:
SBRS1897
Product Type:
ELISA Kit
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human/Mouse/Rat Phospho-MKK3 (S189) and Total MKK3 PharmaGenie ELISA Kit (SBRS1897)

Assay Genie Phospho-MKK3 (S189) and Total MKK3 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated MKK3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-MKK3 and total MKK3. An anti-pan MKK3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-MKK3 (S189) antibody is used to detect phosphorylated MKK3 or biotinylated anti-MKK3 antibody is used to detect pan MKK3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MKK3 (S189) or pan MKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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