Human/Mouse/Rat CD47 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [MIAP410](AGEL0686)

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SKU:
AGEL0686
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Mouse
Isotype:
IgG2a,k
Clone:
MIAP410
Conjugation:
PerCP/Cy5.5

Description

Antibody Name:Human/Mouse/Rat CD47 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated)
Antibody Code:AGEL0686
Size:50µg
Clone:MIAP410
Synonyms:Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Host Species:Mouse
Species Reactivity:Human, Mouse, Rat
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:PerCP/Cyanine5.5
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:961,16423,29364
Uniprot:Q08722, Q61735, P97829
Background:CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Human/Mouse/Rat CD47 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)[Used at ?g/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
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