](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/11946/16000/humanmouserat-cd47-monoclonal-antibody-pecyanine5.5-conjugated-miap410agel0685__49262__74801.1706269764.jpg?c=1&imbypass=on "Human/Mouse/Rat CD47 Monoclonal Antibody (PE/Cyanine5.5 Conjugated) [MIAP410](AGEL0685)")
Description
| Antibody Name: | Human/Mouse/Rat CD47 Monoclonal Antibody (PE/Cyanine5.5 Conjugated) |
| Antibody Code: | AGEL0685 |
| Size: | 50µg |
| Clone: | MIAP410 |
| Synonyms: | Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP |
| Host Species: | Mouse |
| Species Reactivity: | Human, Mouse, Rat |
| Applications: | FC |
| Concentration: | 1mg/mL |
| Isotype: | IgG2a,k |
| Conjugation: | PE/Cyanine5.5 |
| Applications: | FC |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Gene ID: | 961,16423,29364 |
| Uniprot: | Q08722, Q61735, P97829 |
| Background: | CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPĪ±, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking. |
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