](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/11942/15996/humanmouserat-cd47-monoclonal-antibody-apc-conjugated-miap410agel0681__57223__72999.1706269763.jpg?c=1&imbypass=on "Human/Mouse/Rat CD47 Monoclonal Antibody (APC Conjugated) [MIAP410](AGEL0681)")
Description
| Antibody Name: | Human/Mouse/Rat CD47 Monoclonal Antibody (APC Conjugated) |
| Antibody Code: | AGEL0681 |
| Size: | 50µg |
| Clone: | MIAP410 |
| Synonyms: | Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP |
| Host Species: | Mouse |
| Species Reactivity: | Human, Mouse, Rat |
| Applications: | FC |
| Concentration: | 1mg/mL |
| Isotype: | IgG2a,k |
| Conjugation: | APC |
| Applications: | FC |
| Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Gene ID: | 961,16423,29364 |
| Uniprot: | Q08722, Q61735, P97829 |
| Background: | CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking. |
 | C57BL/6 murine splenocytes are stained with Anti-Human/Mouse/Rat CD47 Monoclonal Antibody(APC Conjugated)[Used at ?g/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |
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