Human/Mouse Phospho-MARCKS (S152/156) and Total MARCKS PharmaGenie ELISA Kit (SBRS1888)

(No reviews yet) Write a Review
SKU:
SBRS1888
Product Type:
ELISA Kit
Reactivity:
Human
Reactivity:
Mouse
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human/Mouse Phospho-MARCKS (S152/156) and Total MARCKS PharmaGenie ELISA Kit (SBRS1888)

Assay Genie Phospho-MARCKS (S152/S156) and Total MARCKS ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human and Mouse cell lysates. By determining MARCKS protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human and Mouse phospho-MARCKS and total MARCKS. An anti-pan MARCKS antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MARCKS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-MARCKS (S152/S156) antibody is used to detect phosphorylated MARCKS or biotinylated anti-MARCKS antibody is used to detect pan MARCKS. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MARCKS (S152/S156) or pan MARCKS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

View AllClose

0 Reviews

View AllClose