Enzymes Recombinant Proteins

Human MIOX Recombinant Protein (RPPB1946)

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SKU:
RPPB1946
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UGB7
Research Area:
Enzymes

Description

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Product Name:Human MIOX Recombinant Protein
Product Code:RPPB1946
Size:10µg
Species:Human
Target:MIOX
Synonyms:Myo-Inositol Oxygenase, Kidney-Specific Protein 32, Aldehyde Reductase (Aldose Reductase) Like 6, Renal-Specific Oxidoreductase, Aldehyde Reductase-Like 6, MI Oxygenase, EC 1.13.99.1, ALDRL6, Inositol Oxygenase, KSP32, RSOR, MIOX.
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:MIOX was filtered (0.4 �m) and lyophilized in 20mM Tris buffer, 50mM NaCl and 5% (w/v) trehalose, pH 7.5.
Solubility:It is recommended to add 200�l of deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. MIOX is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHASMKVTVGPDPS LVYRPDVDPE VAKDKASFRN YTSGPLLDRV FTTYKLMHTH QTVDFVRSKH AQFGGFSYKK MTVMEAVDLL DGLVDESDPD VDFPNSFHAF QTAEGIRKAH PDKDWFHLVG LLHDLGKVLA LFGEPQWAVV GDTFPVGCRP QASVVFCDST FQDNPDLQDP RYSTELGMYQ PHCGLDRVLM SWGHDEYMYQ VMKFNKFSLP PEAFYMIRFH SFYPWHTGRD YQQLCSQQDL AMLPWVREFN KFDLYTKCPD LPDVDKLRPY YQGLIDKYCP GILSW

Inositol oxygenase is a non-heme di-iron enzyme which oxidizes myo-inositol to glucuronic acid. In addition, inositol oxygenase oxidizes the less abundant chiro isomer of inositol. MIOX enzyme is a component of the only known pathway for the catabolism of inositol in humans. MIOX is expressed mostly in the kidneys. Reduction of Inositol Oxygenase and accumulation of polyols, such as inositol and xylitol, have been implicated as contributing factors in complications linked with diabetes.

MIOX Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Met1-Trp285) containing 295 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 34.2kDa.

UniProt Protein Function:MIOX: Belongs to the myo-inositol oxygenase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.13.99.1; Carbohydrate Metabolism - ascorbate and aldarate; Oxidoreductase; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 22q13.3

Cellular Component: cytoplasm; inclusion body

Molecular Function:aldo-keto reductase activity; ferric iron binding; inositol oxygenase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen

Biological Process: inositol catabolic process

UniProt Code:Q9UGB7
NCBI GenInfo Identifier:28380090
NCBI Gene ID:55586
NCBI Accession:Q9UGB7.1
UniProt Secondary Accession:Q9UGB7,Q05DJ6, Q5S8C9, Q9BZZ1, Q9UHB8,
UniProt Related Accession:Q9UGB7
Molecular Weight:24,516 Da
NCBI Full Name:Inositol oxygenase
NCBI Synonym Full Names:myo-inositol oxygenase
NCBI Official Symbol:MIOX��
NCBI Official Synonym Symbols:ALDRL6��
NCBI Protein Information:inositol oxygenase
UniProt Protein Name:Inositol oxygenase
UniProt Synonym Protein Names:Aldehyde reductase-like 6; Kidney-specific protein 32; Myo-inositol oxygenase; MI oxygenase; Renal-specific oxidoreductase
Protein Family:Inositol oxygenase
UniProt Gene Name:MIOX��
UniProt Entry Name:MIOX_HUMAN
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