Growth Factors & Cytokines Recombinant Proteins

Human MIF Recombinant Protein (RPPB0763)

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SKU:
RPPB0763
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P14174
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Human MIF Recombinant Protein
Product Code:RPPB0763
Size:20µg
Species:Human
Target:MIF
Synonyms:Macrophage Migration Inhibitory Factor(Glycosylation-Inhibiting Factor), Phenylpyruvate Tautomerase, L-DopachromeTautomerase, L-Dopachrome Isomerase, GLIF, MMIF, GIF, MacrophageMigration Inhibitory Factor, Glycosylation-Inhibiting Factor, EC 5.3.3.12, EC5.3.2.1, Macrophage migration inhibitory factor.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:MIF protein solution (1mg/ml) containing PhosphateBuffered Saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MSPILGYWKIKGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHNMLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSDLVPRGSPEFAMPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGGAQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

MIF Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 345 amino acids (1-115 a.a) andhaving a molecular mass of 39.2kDa.MIF is fused to a 230 aminoacid GST-tag at N-terminus & purified by proprietary chromatographictechniques.

UniProt Protein Function:MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.
UniProt Protein Details:

Protein type:EC 5.3.2.1; Isomerase; EC 5.3.3.12; Amino Acid Metabolism - tyrosine; Cell development/differentiation; Cytokine; Apoptosis; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle

Molecular Function:chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding

Biological Process: blood coagulation; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation

Disease: Rheumatoid Arthritis, Systemic Juvenile

NCBI Summary:This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
UniProt Code:P14174
NCBI GenInfo Identifier:1170955
NCBI Gene ID:4282
NCBI Accession:P14174.4
UniProt Secondary Accession:P14174,Q2V4Y5, Q6FHV0, A5Z1R8, B2R4S3,
UniProt Related Accession:P14174
Molecular Weight:12,476 Da
NCBI Full Name:Macrophage migration inhibitory factor
NCBI Synonym Full Names:macrophage migration inhibitory factor (glycosylation-inhibiting factor)
NCBI Official Symbol:MIF��
NCBI Official Synonym Symbols:GIF; GLIF; MMIF��
NCBI Protein Information:macrophage migration inhibitory factor
UniProt Protein Name:Macrophage migration inhibitory factor
UniProt Synonym Protein Names:Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
Protein Family:MIF-like protein
UniProt Gene Name:MIF��
UniProt Entry Name:MIF_HUMAN
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