Neurotrophins Recombinant Proteins
Human Midkine Recombinant Protein (RPPB0771)
- SKU:
- RPPB0771
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21741
- Research Area:
- Neurotrophins
Description
| Product Name: | Human Midkine Recombinant Protein |
| Product Code: | RPPB0771 |
| Size: | 20µg |
| Species: | Human |
| Target: | Midkine |
| Synonyms: | NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 1�PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Midkine in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Midkine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD |
| Biological Activity: | Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10 ng/ml corresponding to a specific activity of 100,000-10,000,000IU/mg. |
Midkine (MK) is the product of a retinoic acid responsive gene. It contains 121 amino acid residues including 10 conserved cysteine residues, all of which appear to be disulphide linked.Midkine is expressed during embryogenesis, showing an expression pattern that suggests functions in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm-epithelial interaction.The widespread downregulation of MK in the adult human is reverted in a number of cancers, in which polypeptides are able to act as both transforming growth factors and promoters of angiogenesis.Midkine (MK), induces chemotaxis of human neutrophils and was found to trigger mobilization of intracellular calcium of these cells.Midkine induces histamine release from rat peritoneal mast cells with a rapid response in a dose dependent manner.Midkine is also a potent stimulator of collagen and glycosaminoglycan synthesis.
Midkine Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 123 amino acids and having a molecular mass of 13.4kDa. The Midkine is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: cell projection; cytoplasm; extracellular region Molecular Function:heparin binding; growth factor activity Biological Process: response to drug; nervous system development; cell migration; Notch signaling pathway; dentate gyrus development; behavioral fear response; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior |
| NCBI Summary: | This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012] |
| UniProt Code: | P21741 |
| NCBI GenInfo Identifier: | 127116 |
| NCBI Gene ID: | 4192 |
| NCBI Accession: | P21741.1 |
| UniProt Secondary Accession: | P21741,Q2LEK4, Q9UCC7, |
| UniProt Related Accession: | P21741 |
| Molecular Weight: | 9,512 Da |
| NCBI Full Name: | Midkine |
| NCBI Synonym Full Names: | midkine (neurite growth-promoting factor 2) |
| NCBI Official Symbol: | MDK�� |
| NCBI Official Synonym Symbols: | MK; ARAP; NEGF2�� |
| NCBI Protein Information: | midkine; retinoic acid inducible factor; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting factor 2 |
| UniProt Protein Name: | Midkine |
| UniProt Synonym Protein Names: | Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein |
| Protein Family: | Midkine |
| UniProt Gene Name: | MDK�� |
| UniProt Entry Name: | MK_HUMAN |
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