Enzymes Recombinant Proteins
Human MGLL Recombinant Protein (RPPB1944)
- SKU:
- RPPB1944
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q99685
- Research Area:
- Enzymes
Description
| Product Name: | Human MGLL Recombinant Protein |
| Product Code: | RPPB1944 |
| Size: | 5µg |
| Species: | Human |
| Target: | MGLL |
| Synonyms: | Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL, HUK5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MGLL solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSHMETGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDLLVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFAGMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFGIQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTNSVFHEINMWV SQRTATAGTA SPP |
| Biological Activity: | Specificactivity is > 170 units/mg, and is defined as the amount of enzyme that hydrolyze1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at25C. |
MGLL is a membrane-associated member of the serine hydrolase superfamily. MGLL is expressed in abundance in skeletal muscle and adipose tissue. MGLL functions jointly with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides.
MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. |
| UniProt Protein Details: | Protein type:Phospholipase; EC 3.1.1.23; Lipid Metabolism - glycerolipid Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol Molecular Function:protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; triacylglycerol catabolic process; regulation of inflammatory response; arachidonic acid metabolic process; lipid metabolic process; regulation of sensory perception of pain; inflammatory response; blood coagulation; fatty acid biosynthetic process; acylglycerol catabolic process |
| NCBI Summary: | This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q99685 |
| NCBI GenInfo Identifier: | 47117287 |
| NCBI Gene ID: | 11343 |
| NCBI Accession: | Q99685.2 |
| UniProt Secondary Accession: | Q99685,Q6IBG9, Q96AA5, B3KRC2, B7Z9D1, |
| UniProt Related Accession: | Q99685 |
| Molecular Weight: | 303 |
| NCBI Full Name: | Monoglyceride lipase |
| NCBI Synonym Full Names: | monoglyceride lipase |
| NCBI Official Symbol: | MGLL�� |
| NCBI Official Synonym Symbols: | MGL; HUK5; MAGL; HU-K5�� |
| NCBI Protein Information: | monoglyceride lipase; monoacylglycerol lipase; lysophospholipase homolog |
| UniProt Protein Name: | Monoglyceride lipase |
| UniProt Synonym Protein Names: | HU-K5; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MAGL |
| Protein Family: | Monoglyceride lipase |
| UniProt Gene Name: | MGLL�� |
| UniProt Entry Name: | MGLL_HUMAN |
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