Enzymes Recombinant Proteins

Human MGLL Recombinant Protein (RPPB1943)

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SKU:
RPPB1943
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q99685
Research Area:
Enzymes

Description

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Product Name:Human MGLL Recombinant Protein
Product Code:RPPB1943
Size:10µg
Species:Human
Target:MGLL
Synonyms:Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL, HUK5.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The MGLL solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP

MGLL is a membrane-associated member of the serine hydrolase superfamily. MGLL is expressed in abundance in skeletal muscle and adipose tissue. MGLL functions jointly with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides.

MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.

UniProt Protein Function:MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
UniProt Protein Details:

Protein type:Phospholipase; EC 3.1.1.23; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol

Molecular Function:protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity

Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; triacylglycerol catabolic process; regulation of inflammatory response; arachidonic acid metabolic process; lipid metabolic process; regulation of sensory perception of pain; inflammatory response; blood coagulation; fatty acid biosynthetic process; acylglycerol catabolic process

NCBI Summary:This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:Q99685
NCBI GenInfo Identifier:47117287
NCBI Gene ID:11343
NCBI Accession:Q99685.2
UniProt Secondary Accession:Q99685,Q6IBG9, Q96AA5, B3KRC2, B7Z9D1,
UniProt Related Accession:Q99685
Molecular Weight:303
NCBI Full Name:Monoglyceride lipase
NCBI Synonym Full Names:monoglyceride lipase
NCBI Official Symbol:MGLL��
NCBI Official Synonym Symbols:MGL; HUK5; MAGL; HU-K5��
NCBI Protein Information:monoglyceride lipase; monoacylglycerol lipase; lysophospholipase homolog
UniProt Protein Name:Monoglyceride lipase
UniProt Synonym Protein Names:HU-K5; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MAGL
Protein Family:Monoglyceride lipase
UniProt Gene Name:MGLL��
UniProt Entry Name:MGLL_HUMAN
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