Human MED4 Recombinant Protein (RPPB3951)

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SKU:
RPPB3951
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NPJ6

Description

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Product Name:Human MED4 Recombinant Protein
Product Code:RPPB3951
Size:25µg
Species:Human
Target:MED4
Synonyms:Mediator of RNA polymerase II transcription subunit 4, Mediator complex subunit 4, Vitamin D3 receptor-interacting protein complex 36 kDa component, Activator-recruited cofactor 36 kDa component, TRAP/SMCC/PC2 subunit p36 subunit, DRIP36, ARC36, MED4, VDRIP, TRAP36, HSPC126, FLJ10956, RP11-90M2.2.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The MED4 protein solution contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 1mM DTT and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH

Mediator complex subunit 4 (MED4) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED4 is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is able to activate nuclear receptors in a ligand-dependent manner. MED4 functions as a link to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription apparatus. MED4 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

MED4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 278 amino acids (1-270 a.a.) and having a molecular mass of 30.7kDa.MED4 is fused to 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:MED4: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 4 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus

Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; gene expression

NCBI Summary:This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:Q9NPJ6
NCBI GenInfo Identifier:29840770
NCBI Gene ID:29079
NCBI Accession:Q9NPJ6.1
UniProt Secondary Accession:Q9NPJ6,Q53GB4, Q53H68, Q5T912, Q6FHC4, Q6IA79, Q9BS95 Q9NYR5, B4DX67,
UniProt Related Accession:Q9NPJ6
Molecular Weight:24,947 Da
NCBI Full Name:Mediator of RNA polymerase II transcription subunit 4
NCBI Synonym Full Names:mediator complex subunit 4
NCBI Official Symbol:MED4��
NCBI Official Synonym Symbols:ARC36; VDRIP; DRIP36; TRAP36; HSPC126��
NCBI Protein Information:mediator of RNA polymerase II transcription subunit 4; TRAP/SMCC/PC2 subunit p36; mediator, 34-kD subunit, homolog; activator-recruited cofactor 36 kDa component; vitamin D receptor-interacting protein, 36-kD; vitamin D3 receptor-interacting protein complex 36 kDa component
UniProt Protein Name:Mediator of RNA polymerase II transcription subunit 4
UniProt Synonym Protein Names:Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36
Protein Family:Mediator of RNA polymerase II transcription
UniProt Gene Name:MED4��
UniProt Entry Name:MED4_HUMAN
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