Human MED20 Recombinant Protein (RPPB3950)

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SKU:
RPPB3950
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H944

Description

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Product Name:Human MED20 Recombinant Protein
Product Code:RPPB3950
Size:20µg
Species:Human
Target:MED20
Synonyms:Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP, PRO0213.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:MED20 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 50% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR

Mediator Complex Subunit 20 (MED20) is a subunit of the Mediator complex, which is a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors. The Mediator functions as a link to transmit information from gene-specific regulatory proteins to the basal RNA polymerase II transcription apparatus. Mediator is recruited to promoters by direct interactions with regulatory proteins and functions as a scaffold for the compilation of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

MED20 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (1-212 a.a.) and having a molecular mass of 25kDa.MED20 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:TRFP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 20 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; Srb-mediator complex

Molecular Function:protein binding; DNA-directed RNA polymerase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; gene expression

NCBI Summary:MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]
UniProt Code:Q9H944
NCBI GenInfo Identifier:30023851
NCBI Gene ID:9477
NCBI Accession:NP_004266.2
UniProt Secondary Accession:Q9H944,O95821, Q5T8J4, Q9Y429, B4DE08,
UniProt Related Accession:Q9H944
Molecular Weight:13,540 Da
NCBI Full Name:mediator of RNA polymerase II transcription subunit 20
NCBI Synonym Full Names:mediator complex subunit 20
NCBI Official Symbol:MED20��
NCBI Official Synonym Symbols:TRFP; PRO0213��
NCBI Protein Information:mediator of RNA polymerase II transcription subunit 20; TRF-proximal protein homolog; Trf (TATA binding protein-related factor)-proximal homolog; hTRFP; mediator complex subunit 20mediator complex subunit 20mediator complex subunit 20Trf (TATA binding protein-related factor)-proximal homolog
UniProt Protein Name:Mediator of RNA polymerase II transcription subunit 20
UniProt Synonym Protein Names:Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP
Protein Family:Putative mediator of RNA polymerase II transcription
UniProt Gene Name:MED20��
UniProt Entry Name:MED20_HUMAN
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