Enzymes Recombinant Proteins

Human MAT2A Recombinant Protein (RPPB1925)

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RPPB1925

Description

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Product Name:Human MAT2A Recombinant Protein
Product Code:RPPB1925
Size:20µg
Species:Human
Target:MAT2A
Synonyms:MATA2, MATII, SAMS2, MAT-2A, S-adenosylmethionine synthetase isoform type-2, AdoMet synthetase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT2A, AMS2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The MAT2A solution (1mg/ml) contains 20mM Tris pH-8 & 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MNGQLNGFHE AFIEEGTFLF TSESVGEGHP DKICDQISDA VLDAHLQQDP DAKVACETVA KTGMILLAGE ITSRAAVDYQ KVVREAVKHI GYDDSSKGFD YKTCNVLVAL EQQSPDIAQG VHLDRNEEDI GAGDQGLMFG YATDETEECM PLTIVLAHKL NAKLAELRRN GTLPWLRPDS KTQVTVQYMQ DRGAVLPIRV HTIVISVQHD EEVCLDEMRD ALKEKVIKAV VPAKYLDEDT IYHLQPSGRF VIGGPQGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKGGL CRRVLVQVSY AIGVSHPLSI SIFHYGTSQK SERELLEIVK KNFDLRPGVI VRDLDLKKPI YQRTAAYGHF GRDSFPWEVP KKLKY

MAT2A is an important enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer. NF-kappa B and AP-1 are necessary for basal MAT2A expression in HepG2 cells and mediate the increase in MAT2A expression in response to TNF-alpha. Up-regulation of MAT2A provides growth improvement and s-adenosylmethionine and methylthioadenosine thus can block mitogenic signaling in colon cancer cells. Lower expression of both MAT2A and MAT2beta and interfere with leptin signaling in liver cancer cells.

MAT2A Human Recombinant fused with His tag (20 a.a.) at C-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 415 amino acids and having a molecular mass of 45.8 kDa.The MAT2A is purified by proprietary chromatographic techniques.

UniProt Protein Function:MAT2A: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Belongs to the AdoMet synthase family.
UniProt Protein Details:

Protein type:Transferase; Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine; EC 2.5.1.6

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytosol; methionine adenosyltransferase complex

Molecular Function:methionine adenosyltransferase activity; protein binding

Biological Process: methylation; S-adenosylmethionine biosynthetic process

NCBI Summary:The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]
UniProt Code:P31153
NCBI GenInfo Identifier:400245
NCBI Gene ID:4144
NCBI Accession:P31153.1
UniProt Secondary Accession:P31153,Q53SP5, A8K511, B4DN45, D6W5L1,
UniProt Related Accession:P31153
Molecular Weight:32,965 Da
NCBI Full Name:S-adenosylmethionine synthase isoform type-2
NCBI Synonym Full Names:methionine adenosyltransferase 2A
NCBI Official Symbol:MAT2A��
NCBI Official Synonym Symbols:MATA2; MATII; SAMS2��
NCBI Protein Information:S-adenosylmethionine synthase isoform type-2
UniProt Protein Name:S-adenosylmethionine synthase isoform type-2
UniProt Synonym Protein Names:Methionine adenosyltransferase 2; MAT 2; Methionine adenosyltransferase II; MAT-II
Protein Family:S-adenosylmethionine synthase
UniProt Gene Name:MAT2A��
UniProt Entry Name:METK2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P31153
Research Area:
Enzymes
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