Enzymes Recombinant Proteins
Human MAT1A Recombinant Protein (RPPB1924)
- SKU:
- RPPB1924
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q00266
- Research Area:
- Enzymes
Description
| Product Name: | Human MAT1A Recombinant Protein |
| Product Code: | RPPB1924 |
| Size: | 10µg |
| Species: | Human |
| Target: | MAT1A |
| Synonyms: | EC 2.5.1.6, MAT, MATA1, SAMS, SAMS1, Methionine adenosyltransferase 1, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, MAT 1, Methionine adenosyltransferase I/III, MAT-I/III, MAT1A, AMS1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MAT1A protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, 100mM NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHS SGLVPRGSHM NGPVDGLCDH SLSEGVFMFT SESVGEGHPD KICDQISDAV LDAHLKQDPN AKVACETVCK TGMVLLCGEI TSMAMVDYQR VVRDTIKHIG YDDSAKGFDF KTCNVLVALE QQSPDIAQCV HLDRNEEDVG AGDQGLMFGY ATDETEECMP LTIILAHKLN ARMADLRRSG LLPWLRPDSK TQVTVQYMQD NGAVIPVRIH TIVISVQHNE DITLEEMRRA LKEQVIRAVV PAKYLDEDTV YHLQPSGRFV IGGPQGDAGV TGRKIIVDTY GGWGAHGGGA FSGKDYTKVD RSAAYAARWV AKSLVKAGLC RRVLVQVSYA IGVAEPLSIS IFTYGTSQKT ERELLDVVHK NFDLRPGVIV RDLDLKKPIY QKTACYGHFG RSEFPWEVPR KLVF |
MAT1A catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. MAT1A is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in MAT1A gene are associated with methionine adenosyltransferase deficiency. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NF?B and TNF? cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth.
MAT1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 414 amino acids (1-395 a.a.) and having a molecular mass of 45.6 kDa. The MAT1A is fused to a 20 amino acid his tag at N-terminus and purified by conventional chromatography.
| UniProt Protein Function: | MAT1A: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Defects in MAT1A are the cause of methionine adenosyltransferase deficiency (MATD); also called MAT I/III deficiency. MATD is an inborn error of metabolism resulting in isolated hypermethioninemia. Most patients have no clinical abnormalities, although some neurologic symptoms may be present in rare cases with severe loss of methionine adenosyltransferase activity. Belongs to the AdoMet synthase family. |
| UniProt Protein Details: | Protein type:EC 2.5.1.6; Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine; Transferase Chromosomal Location of Human Ortholog: 10q22 Cellular Component: cytosol Molecular Function:methionine adenosyltransferase activity Biological Process: amino acid metabolic process; methylation; selenium metabolic process; sulfur amino acid metabolic process Disease: Methionine Adenosyltransferase Deficiency |
| NCBI Summary: | This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q00266 |
| NCBI GenInfo Identifier: | 417297 |
| NCBI Gene ID: | 4143 |
| NCBI Accession: | Q00266.2 |
| UniProt Secondary Accession: | Q00266,Q5QP09, D3DWD5, |
| UniProt Related Accession: | Q00266 |
| Molecular Weight: | 43,648 Da |
| NCBI Full Name: | S-adenosylmethionine synthase isoform type-1 |
| NCBI Synonym Full Names: | methionine adenosyltransferase 1A |
| NCBI Official Symbol: | MAT1A�� |
| NCBI Official Synonym Symbols: | MAT; SAMS; MATA1; SAMS1�� |
| NCBI Protein Information: | S-adenosylmethionine synthase isoform type-1 |
| UniProt Protein Name: | S-adenosylmethionine synthase isoform type-1 |
| UniProt Synonym Protein Names: | Methionine adenosyltransferase 1; MAT 1; Methionine adenosyltransferase I/III; MAT-I/III |
| Protein Family: | S-adenosylmethionine synthase |
| UniProt Gene Name: | MAT1A�� |
| UniProt Entry Name: | METK1_HUMAN |
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