Enzymes Recombinant Proteins
Human MAPKAPK3 Recombinant Protein (RPPB2581)
- SKU:
- RPPB2581
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q16644
- Research Area:
- Enzymes
Description
| Product Name: | Human MAPKAPK3 Recombinant Protein |
| Product Code: | RPPB2581 |
| Size: | 20µg |
| Species: | Human |
| Target: | MAPKAPK3 |
| Synonyms: | 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3,MAPKAPK-3, MK-3,MAPKAPK3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MAPKAPK3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDGETAE EQGGPVPPPV APGGPGLGGA PGGRREPKKY AVTDDYQLSK QVLGLGVNGK VLECFHRRTG QKCALKLLYD SPKARQEVDH HWQASGGPHI VCILDVYENM HHGKRCLLII MECMEGGELF SRIQERGDQA FTEREAAEIM RDIGTAIQFL HSHNIAHRDV KPENLLYTSK EKDAVLKLTD FGFAKETTQN ALQTPCYTPY YVAPEVLGPE KYDKSCDMWS LGVIMYILLC GFPPFYSNTG QAISPGMKRR IRLGQYGFPN PEWSEVSEDA KQLIRLLLKT DPTERLTITQ FMNHPWINQS MVVPQTPLHT ARVLQEDKDH WDEVKEEMTS ALATMRVDYD QVKIKDLKTS NNRLLNKRRK KQAGSSSASQ GCNNQ |
MAP kinase-activated protein kinase 3 (MAPKAPK3) is involved in inflammatory Reaction by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally. MAPKAPK3 phosphorylates AU-rich elements (AREs)-binding proteins, like TTP/ZFP36, leading to control of stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36 (a major post-transcriptional regulator of TNF), promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity resulting in inhibition of dependent degradation of ARE-containing transcript. MAPKAPK3 is activated by growth inducers and stress stimulation of cells.
MAPKAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 405 amino acids (1-382 a.a) and having a molecular mass of 45.4kDa. MAPKAPK3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MAPKAPK3: a member of the MAPKAPK family of protein kinases. Activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. |
| UniProt Protein Details: | Protein type:Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; MAPKAPK family; MAPKAPK subfamily Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; MAP kinase kinase activity; protein binding; protein serine/threonine kinase activity; signal transducer activity Biological Process: activation of MAPK activity; cell surface receptor linked signal transduction; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; Ras protein signal transduction; response to cytokine stimulus; response to lipopolysaccharide; response to stress; signal transduction; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q16644 |
| NCBI GenInfo Identifier: | 74762148 |
| NCBI Gene ID: | 7867 |
| NCBI Accession: | Q16644.1 |
| UniProt Secondary Accession: | Q16644,B5BU67, |
| UniProt Related Accession: | Q16644 |
| Molecular Weight: | |
| NCBI Full Name: | MAP kinase-activated protein kinase 3 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase-activated protein kinase 3 |
| NCBI Official Symbol: | MAPKAPK3�� |
| NCBI Official Synonym Symbols: | 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3�� |
| NCBI Protein Information: | MAP kinase-activated protein kinase 3 |
| UniProt Protein Name: | MAP kinase-activated protein kinase 3 |
| UniProt Synonym Protein Names: | Chromosome 3p kinase; 3pK |
| Protein Family: | MAP kinase-activated protein kinase |
| UniProt Gene Name: | MAPKAPK3�� |
| UniProt Entry Name: | MAPK3_HUMAN |
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