Human MAP1LC3A Recombinant Protein (RPPB3920)
- SKU:
- RPPB3920
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9H492
Description
| Product Name: | Human MAP1LC3A Recombinant Protein |
| Product Code: | RPPB3920 |
| Size: | 20µg |
| Species: | Human |
| Target: | MAP1LC3A |
| Synonyms: | ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, MLP3A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The MAP1LC3A solution (1mg/ml) contains 20mM Tris-HCl pH-8.0, 1mM DTT, 0.1M NaCl & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG LEHHHHHH |
MAP1LC3A takes part in formation of autophagosomal vacuoles and is one of the light chain subunits that functions together with both MAP1A and/or MAP1B. MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
MAP1LC3A Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 128 amino acids (1-120 a.a.) and having a molecular mass of 15.1 kDa. The MAP1LC3A is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Microtubule-binding; Ubiquitin-like modifier; Autophagy; Vesicle Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol Molecular Function:protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation |
| NCBI Summary: | MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q9H492 |
| NCBI GenInfo Identifier: | 85701362 |
| NCBI Gene ID: | 84557 |
| NCBI Accession: | Q9H492.2 |
| UniProt Secondary Accession: | Q9H492,Q9GZQ8, |
| UniProt Related Accession: | Q9H492 |
| Molecular Weight: | |
| NCBI Full Name: | Microtubule-associated proteins 1A/1B light chain 3A |
| NCBI Synonym Full Names: | microtubule associated protein 1 light chain 3 alpha |
| NCBI Official Symbol: | MAP1LC3A�� |
| NCBI Official Synonym Symbols: | LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3�� |
| NCBI Protein Information: | microtubule-associated proteins 1A/1B light chain 3A |
| UniProt Protein Name: | Microtubule-associated proteins 1A/1B light chain 3A |
| UniProt Synonym Protein Names: | Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha |
| UniProt Gene Name: | MAP1LC3A�� |
| UniProt Entry Name: | MLP3A_HUMAN |
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