Neurotrophins Recombinant Proteins
Human MANF Recombinant Protein (RPPB0745)
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- SKU:
- RPPB0745
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P55145
- Research Area:
- Neurotrophins
Description
| Product Name: | Human MANF Recombinant Protein |
| Product Code: | RPPB0745 |
| Size: | 20µg |
| Species: | Human |
| Target: | MANF |
| Synonyms: | Mesencephalic astrocyte-derived neurotrophic factor, Protein ARMET, ARP, arginine-rich mutated in early stage tumors, Arginine-rich protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.2�m) and lyophilized from a concentrated (1mg/ml) solution in 1�PBS, pH7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized MANF in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized MANF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MANF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL |
| Biological Activity: | The ED50 was determined by its ability to stimulate the proliferation of rat C6 cells is typically 15-25 �g/ml. |
MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.
MANF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18.1 kDa. The MANF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: nucleus Disease: Pancreatic Cancer |
| NCBI Summary: | The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014] |
| UniProt Code: | P55145 |
| NCBI GenInfo Identifier: | 332278201 |
| NCBI Gene ID: | 7873 |
| NCBI Accession: | P55145.3 |
| UniProt Secondary Accession: | P55145,Q14CX4, Q86U67, Q96IS4, |
| UniProt Related Accession: | P55145 |
| Molecular Weight: | 20,700 Da |
| NCBI Full Name: | Mesencephalic astrocyte-derived neurotrophic factor |
| NCBI Synonym Full Names: | mesencephalic astrocyte derived neurotrophic factor |
| NCBI Official Symbol: | MANF�� |
| NCBI Official Synonym Symbols: | ARP; ARMET�� |
| NCBI Protein Information: | mesencephalic astrocyte-derived neurotrophic factor |
| UniProt Protein Name: | Mesencephalic astrocyte-derived neurotrophic factor |
| UniProt Synonym Protein Names: | Arginine-rich protein; Protein ARMET |
| Protein Family: | MANF/CDNF-like protein |
| UniProt Gene Name: | MANF�� |
| UniProt Entry Name: | MANF_HUMAN |
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