Neurotrophins Recombinant Proteins

Human MANF Recombinant Protein (RPPB0744)

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RPPB0744

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human MANF Recombinant Protein
Product Code:RPPB0744
Size:20µg
Species:Human
Target:MANF
Synonyms:Mesencephalic astrocyte-derived neurotrophic factor, Protein ARMET, ARP, arginine-rich mutated in early stage tumors, Arginine-rich protein.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The MANF solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMLRPGD CEVCISYLGR FYQDLKDRDV TFSPATIENE LIKFCREARG KENRLCYYIG ATDDAATKII NEVSKPLAHH IPVEKICEKL KKKDSQICEL KYDKQIDLST VDLKKLRVKE LKKILDDWGE TCKGCAEKSD YIRKINELMP KYAPKAASAR TDL

MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

MANF Human Recombinant produced in E. coli is a single polypeptide chain containing 183 amino acids (25-182) and having a molecular mass of 20.8kDa.MANF is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleus

Disease: Pancreatic Cancer

NCBI Summary:The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
UniProt Code:P55145
NCBI GenInfo Identifier:332278201
NCBI Gene ID:7873
NCBI Accession:P55145.3
UniProt Secondary Accession:P55145,Q14CX4, Q86U67, Q96IS4,
UniProt Related Accession:P55145
Molecular Weight:20,700 Da
NCBI Full Name:Mesencephalic astrocyte-derived neurotrophic factor
NCBI Synonym Full Names:mesencephalic astrocyte derived neurotrophic factor
NCBI Official Symbol:MANF��
NCBI Official Synonym Symbols:ARP; ARMET��
NCBI Protein Information:mesencephalic astrocyte-derived neurotrophic factor
UniProt Protein Name:Mesencephalic astrocyte-derived neurotrophic factor
UniProt Synonym Protein Names:Arginine-rich protein; Protein ARMET
Protein Family:MANF/CDNF-like protein
UniProt Gene Name:MANF��
UniProt Entry Name:MANF_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P55145
Research Area:
Neurotrophins
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Neurotrophins Recombinant Proteins

system_update_altDatasheetHuman MANF/ARMET Recombinant Protein Mesencephalic astrocyte-derived neurotrophic factor(MANF) is a secreted protein which belongs to the ARMET family. MANF selectively