Human MAGEA3 Recombinant Protein (RPPB3910)

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SKU:
RPPB3910
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P43357

Description

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Product Name:Human MAGEA3 Recombinant Protein
Product Code:RPPB3910
Size:10µg
Species:Human
Target:MAGEA3
Synonyms:CT1.3, MAGE3, HYPD, Melanoma Antigen family A, 3, MAGE-3 antigen, MAGEA6, Antigen MZ2-D, Melanoma-Associated antigen 3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The MAGEA3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 100mM NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMPLEQRS QHCKPEEGLE ARGEALGLVG AQAPATEEQE AASSSSTLVE VTLGEVPAAE SPDPPQSPQG ASSLPTTMNY PLWSQSYEDS SNQEEEGPST FPDLESEFQA ALSRKVAELV HFLLLKYRAR EPVTKAEMLG SVVGNWQYFF PVIFSKASSS LQLVFGIELM EVDPIGHLYI FATCLGLSYD GLLGDNQIMP KAGLLIIVLA IIAREGDCAP EEKIWEELSV LEVFEGREDS ILGDPKKLLT QHFVQENYLE YRQVPGSDPA CYEFLWGPRA LVETSYVKVL HHMVKISGGP HISYPPLHEW VLREGEE

MAGE belongs to the MAGE gene family, that comprises 12 known genes, of which 6 are expressed in tumors. The Melanoma-associated antigen 3 genes were originally isolated from different kinds of tumors, and based on their virtually limited tumor-specific expression in adult tissues, they were used as targets for cancer immunotherapy. MAGEA3 is a tumor-specific antigen extensively expressed in solid and hematologic malignancies, but not in normal tissues, with the exclusion of testis and placenta. Consequently, MAGEA3 is an outstanding candidate tumor antigen.

MAGEA3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-314 a.a.) and having a molecular mass of 37.1kDa.MAGEA3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MAGEA3: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
UniProt Protein Details:

Protein type:Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: endoplasmic reticulum

Molecular Function:protein binding

Biological Process: negative regulation of caspase activity

NCBI Summary:This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]
UniProt Code:P43357
NCBI GenInfo Identifier:1170857
NCBI Gene ID:4102
NCBI Accession:P43357.1
UniProt Secondary Accession:P43357,Q6FHI6,
UniProt Related Accession:P43357
Molecular Weight:34,747 Da
NCBI Full Name:Melanoma-associated antigen 3
NCBI Synonym Full Names:MAGE family member A3
NCBI Official Symbol:MAGEA3��
NCBI Official Synonym Symbols:HIP8; HYPD; CT1.3; MAGE3; MAGEA6��
NCBI Protein Information:melanoma-associated antigen 3
UniProt Protein Name:Melanoma-associated antigen 3
UniProt Synonym Protein Names:Antigen MZ2-D; Cancer/testis antigen 1.3; CT1.3; MAGE-3 antigen
Protein Family:Melanoma-associated antigen
UniProt Gene Name:MAGEA3��
UniProt Entry Name:MAGA3_HUMAN
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