Human MAD2L1 Recombinant Protein (RPPB3903)

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SKU:
RPPB3903
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13257

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human MAD2L1 Recombinant Protein
Product Code:RPPB3903
Size:5µg
Species:Human
Target:MAD2L1
Synonyms:Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:MAD2L1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE VRLRSFTTTI HKVNSMVAYK IPVND

MAD2L1-binding protein (MAD2L1) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is vital for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

MAD2L1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 25.7kDa.MAD2L1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: cytosol; kinetochore; nuclear pore; nucleus; perinuclear region of cytoplasm

Molecular Function:identical protein binding; protein binding; protein homodimerization activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; sister chromatid cohesion

NCBI Summary:MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]
UniProt Code:Q13257
NCBI GenInfo Identifier:12230256
NCBI Gene ID:4085
NCBI Accession:Q13257.1
UniProt Secondary Accession:Q13257,Q53F56, Q548X9, Q6IRW7, Q8IZX3,
UniProt Related Accession:Q13257
Molecular Weight:10,335 Da
NCBI Full Name:Mitotic spindle assembly checkpoint protein MAD2A
NCBI Synonym Full Names:MAD2 mitotic arrest deficient-like 1 (yeast)
NCBI Official Symbol:MAD2L1��
NCBI Official Synonym Symbols:MAD2; HSMAD2��
NCBI Protein Information:mitotic spindle assembly checkpoint protein MAD2A
UniProt Protein Name:Mitotic spindle assembly checkpoint protein MAD2A
UniProt Synonym Protein Names:Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1
Protein Family:MAD2L1-binding protein
UniProt Gene Name:MAD2L1��
UniProt Entry Name:MD2L1_HUMAN
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system_update_altDatasheetHuman MAD2L1/MAD2 Recombinant Protein Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like pro