Enzymes Recombinant Proteins

Human LPCAT1 Recombinant Protein (RPPB1910)

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SKU:
RPPB1910
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8NF37
Research Area:
Enzymes

Description

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Product Name:Human LPCAT1 Recombinant Protein
Product Code:RPPB1910
Size:10µg
Species:Human
Target:LPCAT1
Synonyms:AYTL2, lpcat, PFAAP3, Lysophosphatidylcholine acyltransferase 1, LPC acyltransferase 1, LPCAT-1, LysoPC acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Lyso-PAF acetyltransferase, LysoPAFAT, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The LPCAT1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSSAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD

Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a part of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. LPCAT1 is a key enzyme for remodeling phospholipids, including phosphatidylcholine. LPCAT1 possesses both acyltransferase and acetyltransferase activities and also mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). LPCAT1 presents a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. LPCAT1 synthesizes phosphatidylcholine in pulmonary surfactant and therefore playing an important role in respiratory physiology.

LPCAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 479 amino acids (79-534a.a) and having a molecular mass of 53.4kDa. LPCAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:LPCAT1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
UniProt Protein Details:

Protein type:EC 2.3.1.67; Acetyltransferase; Membrane protein, integral; EC 2.3.1.23; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; lipid particle

Molecular Function:1-alkylglycerophosphocholine O-acetyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkenylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acyltransferase activity; calcium ion binding

Biological Process: retina development in camera-type eye; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of protein catabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; surfactant homeostasis; phospholipid biosynthetic process

NCBI Summary:Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]
UniProt Code:Q8NF37
NCBI GenInfo Identifier:110815902
NCBI Gene ID:79888
NCBI Accession:Q8NF37.2
UniProt Secondary Accession:Q8NF37,Q1HAQ1, Q7Z4G6, Q8N3U7, Q8WUL8, Q9GZW6,
UniProt Related Accession:Q8NF37
Molecular Weight:Calculated MW: 59kDaObserved MW:59kDa
NCBI Full Name:Lysophosphatidylcholine acyltransferase 1
NCBI Synonym Full Names:lysophosphatidylcholine acyltransferase 1
NCBI Official Symbol:LPCAT1��
NCBI Official Synonym Symbols:AYTL2; lpcat; PFAAP3��
NCBI Protein Information:lysophosphatidylcholine acyltransferase 1; LPCAT-1; lysoPAFAT; LPC acyltransferase 1; acyltransferase like 2; acyltransferase-like 2; lysoPC acyltransferase 1; lyso-PAF acetyltransferase; regulated by phosphonoformate; acetyl-CoA:lyso-PAF acetyltransferase; phosphonoformate immuno-associated protein 3; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acetyl-CoA:lyso-platelet-activating factor acetyltransferase
UniProt Protein Name:Lysophosphatidylcholine acyltransferase 1
UniProt Synonym Protein Names:1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.67); Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 2; Phosphonoformate immuno-associated protein 3
Protein Family:Lysophosphatidylcholine acyltransferase
UniProt Gene Name:LPCAT1��
UniProt Entry Name:PCAT1_HUMAN
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