Human LIN28B Recombinant Protein (RPPB3862)

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RPPB3862

Description

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Product Name:Human LIN28B Recombinant Protein
Product Code:RPPB3862
Size:5µg
Species:Human
Target:LIN28B
Synonyms:Lin-28 Homolog B (C. Elegans), Protein Lin-28 Homolog B, CSDD2, Lin-28B, Lin-28.2, FLJ16517.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The LIN28B solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT

Lin28B is a member of the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 mRNA. Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28 is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines.

LIN28B Human Recombinant produced in E. coli is a single polypeptide chain containing 273 amino acids (1-250) and having a molecular mass of 29.5 kDa. LIN28B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:LIN28B: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 6q21

Molecular Function:protein binding; RNA binding

Biological Process: pre-microRNA processing; RNA 3'-end processing

NCBI Summary:The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]
UniProt Code:Q6ZN17
NCBI GenInfo Identifier:74758701
NCBI Gene ID:389421
NCBI Accession:Q6ZN17.1
UniProt Secondary Accession:Q6ZN17,Q5TCM4, A1L165, B2RPN6,
UniProt Related Accession:Q6ZN17
Molecular Weight:19,511 Da
NCBI Full Name:Protein lin-28 homolog B
NCBI Synonym Full Names:lin-28 homolog B
NCBI Official Symbol:LIN28B��
NCBI Official Synonym Symbols:CSDD2��
NCBI Protein Information:protein lin-28 homolog B
UniProt Protein Name:Protein lin-28 homolog B
Protein Family:Protein
UniProt Gene Name:LIN28B��
UniProt Entry Name:LN28B_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q6ZN17
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