Immunology Recombinant Proteins

Human LILRB4/CD85k/ILT3 Recombinant Protein (RPES1214)

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RPES1214

Description

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Human LILRB4/CD85k/ILT3 Recombinant Protein

Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains; a 22 aa transmembrane segment; and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM). Within the ECD; mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4; respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells (DC); monocytes; macrophages; and vascular endothelial cells (EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling; leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn; CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes; DC; and EC.

Product Name:Human LILRB4/CD85k/ILT3 Recombinant Protein (RPES1214)
Product Code:RPES1214
Size:10µg
Species:Human
Expressed Host:Human Cells
Synonyms:Leukocyte immunoglobulin-like receptor subfamily B member 4, Mast cell surface glycoprotein Gp49B, CD85k, Lilrb4, Gp49b
Accession:Q8NHJ6
Sequence:Gln22-Glu259
Fusion tag:C-His
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human LILRB4 is produced by our Mammalian expression system and the target gene encoding Gln22-Glu259 is expressed with a 6His tag at the C-terminus.
Purity:> 95% as determined by reducing SDS-PAGE.
Mol Mass:27.1 kDa
AP Mol Mass:37 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of PBS; pH 7.4.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Research Area:
Immunology
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