Cytokines Recombinant Proteins

Human LIF Recombinant Protein (RPPB0739)

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SKU:
RPPB0739
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P15018
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human LIF Recombinant Protein
Product Code:RPPB0739
Size:10µg
Species:Human
Target:LIF
Synonyms:CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:LIF filtered (0.4 �m) and lyophilized from 0.5mg/ml solution in 20 mM Tris buffer, 20 mM NaCl and 5% w/v trehalose, pH 7.5.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHAS PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.

LIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 23-202) containing 189 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 20.9kDa (calculated).

UniProt Protein Function:LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
NCBI Summary:The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:P15018
NCBI GenInfo Identifier:126279
NCBI Gene ID:3976
NCBI Accession:P15018.1
UniProt Secondary Accession:P15018,Q52LZ2, B2RCW7, B5MC23,
UniProt Related Accession:P15018
Molecular Weight:9,563 Da
NCBI Full Name:Leukemia inhibitory factor
NCBI Synonym Full Names:LIF, interleukin 6 family cytokine
NCBI Official Symbol:LIF��
NCBI Official Synonym Symbols:CDF; DIA; HILDA; MLPLI��
NCBI Protein Information:leukemia inhibitory factor
UniProt Protein Name:Leukemia inhibitory factor
UniProt Synonym Protein Names:Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin
Protein Family:Leukemia inhibitory factor
UniProt Gene Name:LIF��
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