Cytokines Recombinant Proteins

Human LIF Recombinant Protein (RPPB0738)

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SKU:
RPPB0738
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P15018
Research Area:
Cytokines

Description

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Product Name:Human LIF Recombinant Protein
Product Code:RPPB0738
Size:10µg
Species:Human
Target:LIF
Synonyms:Leukemia Inhibitory Factor, Differentiation Inhibitory Activity, Cholinergic Differentiation Factor, Differentiation-Stimulating Factor, Hepatocyte-Stimulating Factor III, Differentiation-Inducing Factor, Melanoma-Derived LPL Inhibitor, Human Interleukin In DA Cells, D Factor, HILDA, MLPLI, Emfilermin, DIA, CDF, Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin.
Source:Sf9 Insect cells
Physical Appearance:Sterilefiltered colorless solution.
Formulation:LIF protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPSPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLAQAFHHHHHH
Biological Activity:Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.5 ng/ml.

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.

LIF Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 189 amino acids (23-202a.a.) and having a molecular mass of 20.8kDa (Molecular size on SDS-PAGE will appear at approximately 18-40kDa). LIF is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
NCBI Summary:The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:P15018
NCBI GenInfo Identifier:126279
NCBI Gene ID:3976
NCBI Accession:P15018.1
UniProt Secondary Accession:P15018,Q52LZ2, B2RCW7, B5MC23,
UniProt Related Accession:P15018
Molecular Weight:9,563 Da
NCBI Full Name:Leukemia inhibitory factor
NCBI Synonym Full Names:LIF, interleukin 6 family cytokine
NCBI Official Symbol:LIF��
NCBI Official Synonym Symbols:CDF; DIA; HILDA; MLPLI��
NCBI Protein Information:leukemia inhibitory factor
UniProt Protein Name:Leukemia inhibitory factor
UniProt Synonym Protein Names:Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin
Protein Family:Leukemia inhibitory factor
UniProt Gene Name:LIF��
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