Enzymes Recombinant Proteins

Human LHPP Recombinant Protein (RPPB1904)

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RPPB1904

Description

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Product Name:Human LHPP Recombinant Protein
Product Code:RPPB1904
Size:10µg
Species:Human
Target:LHPP
Synonyms:Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, LHPP, HDHD2B.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The LHPP solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHP WYASMTGGQQ MGRDLYDDDD KDRWGSHMAP WGKRLAGVRG VLLDISGVLY DSGAGGGTAI AGSVEAVARL KRSRLKVRFC TNESQKSRAE LVGQLQRLGF DISEQEVTAP APAACQILKE QGLRPYLLIH DGVRSEFDQI DTSNPNCVVI ADAGESFSYQ NMNNAFQVLM ELEKPVLISL GKGRYYKETS GLMLDVGPYM KALEYACGIK AEVVGKPSPE FFKSALQAIG VEAHQAVMIG DDIVGDVGGA QRCGMRALQV RTGKFRPSDE HHPEVKADGY VDNLAEAVDL LLQHADK

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) belongs to the HAD-like hydrolase superfamily. LHPP is an exceptional enzyme which hydrolyzes not only oxygen-phosphorus bonds in inorganic pyrophosphate but also nitrogen-phosphorus bonds in phospholysine, phosphohistidine and imidodiphosphate in vitro. LHPP is expressed in the liver, kidney and moderately in the brain.

LHPP Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 307 amino acids (1-270) and having a molecular mass of 33.5kDa.LHPP is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:LHPP: Phosphatase that hydrolyzes imidodiphosphate, 3- phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency. Belongs to the HAD-like hydrolase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Hydrolase; Mitochondrial; EC 3.6.1.1; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 10q26.13

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:inorganic diphosphatase activity; magnesium ion binding; phosphohistidine phosphatase activity; protein homodimerization activity

Biological Process: phosphate metabolic process; protein amino acid dephosphorylation; purine ribonucleoside monophosphate biosynthetic process

UniProt Code:Q9H008
NCBI GenInfo Identifier:158705883
NCBI Gene ID:64077
NCBI Accession:Q9H008.2
UniProt Secondary Accession:Q9H008,Q2TBE9, Q5VUV9, Q5VUW0, B3KP20,
UniProt Related Accession:Q9H008
Molecular Weight:22,696 Da
NCBI Full Name:Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
NCBI Synonym Full Names:phospholysine phosphohistidine inorganic pyrophosphate phosphatase
NCBI Official Symbol:LHPP��
NCBI Official Synonym Symbols:HDHD2B��
NCBI Protein Information:phospholysine phosphohistidine inorganic pyrophosphate phosphatase
UniProt Protein Name:Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Protein Family:Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
UniProt Gene Name:LHPP��
UniProt Entry Name:LHPP_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H008
Research Area:
Enzymes
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