Enzymes Recombinant Proteins

Human LGMN Recombinant Protein (RPPB1902)

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SKU:
RPPB1902
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q99538
Research Area:
Enzymes

Description

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Product Name:Human LGMN Recombinant Protein
Product Code:RPPB1902
Size:10µg
Species:Human
Target:LGMN
Synonyms:Legumain, PRSC1, Protease, Cysteine, 1 (Legumain), Asparaginyl Endopeptidase, Protease, Cysteine 1, EC 3.4.22.34, Cysteine Protease 1, LGMN1, AEP.
Source:Sf9 Insect cells
Physical Appearance:Sterile filtered colorless solution.
Formulation:LGMN protein solution (1mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:VPIDDPEDGG KHWVVIVAGS NGWYNYRHQA DACHAYQIIH RNGIPDEQIV VMMYDDIAYS EDNPTPGIVI NRPNGTDVYQ GVPKDYTGED VTPQNFLAVL RGDAEAVKGI GSGKVLKSGP QDHVFIYFTD HGSTGILVFP NEDLHVKDLN ETIHYMYKHK MYRKMVFYIE ACESGSMMNH LPDNINVYAT TAANPRESSY ACYYDEKRST YLGDWYSVNW MEDSDVEDLT KETLHKQYHL VKSHTNTSHV MQYGNKTIST MKVMQFQGMK RKASSPVPLP PVTHLDLTPS PDVPLTIMKR KLMNTNDLEE SRQLTEEIQR HLDARHLIEK SVRKIVSLLA ASEAEVEQLL SERAPLTGHS CYPEALLHFR THCFNWHSPT YEYALRHLYV LVNLCEKPYP LHRIKLSMDH VCLGHYHHHH HH

�Legumain, also known as LGMN, is a cysteine endopeptidase whichdemonstrates strict specificity for hydrolysis of asparaginyl bonds.Furthermore, LGMN can also cleave aspartyl bonds slowly, in particular underacidic conditions. LGMN plays an essential role in the endosomal/lysosomaldegradation system as the Legumain deficiency causes the accumulation of procathepsins B, H & L, another group of lysosomal cysteine proteases.Furthermore, over expression of LGMN in tumors is important for invasion/metastasis.

LGMN produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (18-433 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 422 amino acids and having a molecular mass of 48.4kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).LGMN is purified by proprietary chromatographic techniques.

UniProt Protein Function:LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.
UniProt Protein Details:

Protein type:EC 3.4.22.34; Protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: lysosomal lumen; lysosome

Molecular Function:cysteine-type endopeptidase activity; peptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process

NCBI Summary:This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]
UniProt Code:Q99538
NCBI GenInfo Identifier:2842759
NCBI Gene ID:5641
NCBI Accession:Q99538.1
UniProt Secondary Accession:Q99538,O00123, Q86TV2, Q86TV3, Q9BTY1,
UniProt Related Accession:Q99538
Molecular Weight:Observed MW: 54kDaCalculated MW: 42kDa/49kDa
NCBI Full Name:Legumain
NCBI Synonym Full Names:legumain
NCBI Official Symbol:LGMN��
NCBI Official Synonym Symbols:AEP; LGMN1; PRSC1��
NCBI Protein Information:legumain
UniProt Protein Name:Legumain
UniProt Synonym Protein Names:Asparaginyl endopeptidase; Protease, cysteine 1
UniProt Gene Name:LGMN��
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