Growth Factors & Cytokines Recombinant Proteins

Human Leptin Recombinant Protein (RPPB0703)

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SKU:
RPPB0703
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P41159
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Human Leptin Recombinant Protein
Product Code:RPPB0703
Size:25µg
Species:Human
Target:Leptin
Synonyms:OB Protein, Obesity Protein, OBS, Obesity factor.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.
Solubility:The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
Stability:Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.Please avoid freeze-thaw cycles.
Purity:Greater than 90.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells.

Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.The Leptin is purified by proprietary chromatographic techniques.

UniProt Protein Function:leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Hormone; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function:peptide hormone receptor binding; growth factor activity; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; positive regulation of luteinizing hormone secretion; negative regulation of appetite; positive regulation of tyrosine phosphorylation of Stat3 protein; response to insulin stimulus; response to vitamin E; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; insulin secretion; glycerol biosynthetic process; energy reserve metabolic process; response to hypoxia; hormone metabolic process; regulation of gluconeogenesis; positive regulation of follicle-stimulating hormone secretion; positive regulation of insulin receptor signaling pathway; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency

NCBI Summary:This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]
UniProt Code:P41159
NCBI GenInfo Identifier:730218
NCBI Gene ID:3952
NCBI Accession:P41159.1
UniProt Secondary Accession:P41159,O15158, Q56A88,
UniProt Related Accession:P41159
Molecular Weight:18,641 Da
NCBI Full Name:Leptin
NCBI Synonym Full Names:leptin
NCBI Official Symbol:LEP��
NCBI Official Synonym Symbols:OB; OBS; LEPD��
NCBI Protein Information:leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse)
UniProt Protein Name:Leptin
UniProt Synonym Protein Names:Obese protein; Obesity factor
Protein Family:Leptin
UniProt Gene Name:LEP��
UniProt Entry Name:LEP_HUMAN
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