Enzymes Recombinant Proteins

Human LDHB Recombinant Protein (RPPB1900)

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SKU:
RPPB1900
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P07195
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human LDHB Recombinant Protein
Product Code:RPPB1900
Size:10µg
Species:Human
Target:LDHB
Synonyms:Lactate Dehydrogenase B, Renal Carcinoma AntigenNY-REN-46, LDH Heart Subunit, EC 1.1.1.27, LDH-B, LDH-H, Epididymis SecretoryProtein Li 281, Testicular Secretory Protein Li 25, Lactate Dehydrogenase HChain, HEL-S-281, EC 1.1.1 , LDHBD, TRG-5.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The LDHB solution (1mg/ml) contains 20mM Tris-HCl (pH 8.0), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90% as determined by SDS-PAGE.
Amino Acid Sequence:MATLKEKLIAPVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVADKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWKLSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMGTDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFLSLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
Biological Activity:Specific activity is >300 units/mg, in which 1 unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per min at pH 7.5 at 37�.

LDHB is part of the lactate dehydrogenase family. LDHB is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LDHB catalyzes the oxidation of hydroxybutyrate, and frequently called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.

LDHB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 334 amino acids (1-334) and having a molecular mass of 36.6kDa.

NCBI Summary:This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
UniProt Code:P07195
NCBI GenInfo Identifier:126041
NCBI Gene ID:3945
NCBI Accession:P07195.2
UniProt Related Accession:P07195
Molecular Weight:~ 37kDa
NCBI Full Name:L-lactate dehydrogenase B chain
NCBI Synonym Full Names:lactate dehydrogenase B
NCBI Official Symbol:LDHB��
NCBI Official Synonym Symbols:LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281��
NCBI Protein Information:L-lactate dehydrogenase B chain
UniProt Protein Name:L-lactate dehydrogenase B chain
UniProt Synonym Protein Names:LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:LDHB��
UniProt Entry Name:LDHB_HUMAN
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system_update_altDatasheetHuman LDH-B/LDHB Recombinant Protein L-Lactate Dehydrogenase B Chain (LDH-B) is a member of the lactate dehydrogenase family that consists of three members, LDH-A, LDH-B