Enzymes Recombinant Proteins

Human LDHA Recombinant Protein (RPPB1893)

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RPPB1893

Description

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Product Name:Human LDHA Recombinant Protein
Product Code:RPPB1893
Size:25µg
Species:Human
Target:LDHA
Synonyms:LDH-A, GSD11, LDH1, LDHM, PIG19, EC 1.1.1.27, lactate dehydrogenase M, LDH-M, LDH-1, L-lactate dehydrogenase A chain, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, Cell proliferation-inducing gene 19 protein, LDHA.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The LDHA protein solution (0.5mg/ml) contains 20mM Tris-HCl pH-8.0, 100mM NaCl and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
Biological Activity:Specific activity is > 20 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37�C.

LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

LDHA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-332 a.a.) and having a molecular mass of 38.8 kDa. The LDHA is fused to a 20 amino acid his tag at N-terminus and purified by conventional chromatography.

NCBI Summary:The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
UniProt Code:P00338
NCBI GenInfo Identifier:126047
NCBI Gene ID:3939
NCBI Accession:P00338.2
UniProt Secondary Accession:P00338,Q53G53, Q6IBM7, Q6ZNV1, Q9UDE8, Q9UDE9, B4DKQ2 B7Z5E3, D3DQY3, F8W819,
UniProt Related Accession:P00338
Molecular Weight:26,712 Da
NCBI Full Name:L-lactate dehydrogenase A chain
NCBI Synonym Full Names:lactate dehydrogenase A
NCBI Official Symbol:LDHA��
NCBI Official Synonym Symbols:LDHM; GSD11; PIG19; HEL-S-133P��
NCBI Protein Information:L-lactate dehydrogenase A chain
UniProt Protein Name:L-lactate dehydrogenase A chain
UniProt Synonym Protein Names:Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:LDHA��
UniProt Entry Name:LDHA_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00338
Research Area:
Enzymes
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