Human LCP2 Recombinant Protein (RPPB3851)

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SKU:
RPPB3851
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13094

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human LCP2 Recombinant Protein
Product Code:RPPB3851
Size:5µg
Species:Human
Target:LCP2
Synonyms:Lymphocyte Cytosolic Protein 2, SH2 Domain-Containing Leukocyte Protein Of 76 KDa, 76 KDa Tyrosine Phosphoprotein, SLP-76 Tyrosine Phosphoprotein, SLP76, Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein Of 76kDa), Lymphocyte Cytosolic Protein 2 (SH2 Domain-Containing Leukocyte Protein Of 76kD), SH2 Domain-Containing Leukocyte Protein Of 76Kd, SLP-76, LCP2.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:LCP2 protein solution (0.25mg/ml) contains 10% glycerol & Phosphate buffered saline (pH7.4).
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPMALRNVP FRSEVLGWDP DSLADYFKKL NYKDCEKAVK KYHIDGARFL NLTENDIQKF PKLRVPILSK LSQEINKNEE RRSIFTRKPQ VPRFPEETES HEEDNGGWSS FEEDDYESPN DDQDGEDDGD YESPNEEEEA PVEDDADYEP PPSNDEEALQ NSILPAKPFP NSNSMYIDRP PSGKTPQQPP VPPQRPMAAL PPPPAGRNHS PLPPPQTNHE EPSRSRNHKT AKLPAPSIDR STKPPLDRSL APFDREPFTL GKKPPFSDKP SIPAGRSLGE HLPKIQKPPL PPTTERHERS SPLPGKKPPV PKHGWGPDRR ENDEDDVHQR PLPQPALLPM SSNTFPSRST KPSPMNPLPS SHMPGAFSES NSSFPQSASL PPYFSQGPSN RPPIRAEGRN FPLPLPNKPR PPSPAEEENS LNEEWYVSYI TRPEAEAALR KINQDGTFLV RDSSKKTTTN PYVLMVLYKD KVYNIQIRYQ KESQVYLLGT GLRGKEDFLS VSDIIDYFRK MPLLLIDGKN RGSRYQCTLT HAAGYPHHHH�HH

LCP2 (lymphocyte cytosolic protein 2) is an intracellular docking protein with a single SH2 recognition domain. LCP2 is implicated in T-cell antigen receptor mediated signaling. Moreover, LCP2 is phosphorylated by ZAP70 which leads to NF-AT as well as IL2 gene activation. B Cell Linker Protein Deficiency is one of the associated diseases with LCP2.

LCP2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (1-533 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 542 amino acids and having a molecular mass of 61.2kDa.LCP2 shows multiple bands between 70-100kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

UniProt Protein Function:SLP76: an adaptor protein involved in signaling through the T cell antigen receptor complex and signaling in other hematopoietic lineages including platelets. Originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation. Platelets deficient in SLP76 do not couple GPVI engagement with the signaling cascade that induces degranulation and aggregation in response to collagen. Contains four domains: a COOH-terminal SH2 domain, a central proline-rich domain, an NH2-terminal sterile {alpha} motif domain, and acidic region that contains three tyrosine phosphorylation motifs: Y112ESP, Y128ESP, and Y145EPP. pY112 and pY128 bind SH2 domains of Vav and Nck. pY145 is recognized by SH2 domains of the Tec family kinases Itk and Btk. Highly expressed in spleen, thymus, peripheral blood leukocytes, mast cells, platelets, and T-cell and monocytic cell lines. Expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytosol

Molecular Function:protein binding

Biological Process: immune response; platelet activation; positive regulation of protein kinase activity; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

NCBI Summary:SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function. [provided by RefSeq, Jul 2008]
UniProt Code:Q13094
NCBI GenInfo Identifier:10720065
NCBI Gene ID:3937
NCBI Accession:Q13094.1
UniProt Secondary Accession:Q13094,Q53XV4, A8KA25,
UniProt Related Accession:Q13094
Molecular Weight:60188 Da
NCBI Full Name:Lymphocyte cytosolic protein 2
NCBI Synonym Full Names:lymphocyte cytosolic protein 2
NCBI Official Symbol:LCP2��
NCBI Official Synonym Symbols:SLP76; SLP-76��
NCBI Protein Information:lymphocyte cytosolic protein 2
UniProt Protein Name:Lymphocyte cytosolic protein 2
UniProt Synonym Protein Names:SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76
Protein Family:Larval cuticle protein
UniProt Gene Name:LCP2��
UniProt Entry Name:LCP2_HUMAN
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system_update_altDatasheetHuman LCP2 Recombinant Protein Lymphocyte cytosolic protein 2?LCP2?contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood