Human KRT14 Recombinant Protein (RPPB3814)

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SKU:
RPPB3814
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P02533

Description

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Product Name:Human KRT14 Recombinant Protein
Product Code:RPPB3814
Size:20µg
Species:Human
Target:KRT14
Synonyms:Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14, NFJ, CK14, EBS3, EBS4.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:KRT14 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIKDYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQNLEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN

Cytokeratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. Cytokeratin 14 is a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

KRT14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 492 amino acids (1-472 a.a.) and having a molecular mass of 53.8kDa.KRT14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; keratin filament; intermediate filament; cytosol; nucleus

Molecular Function:protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; hair cycle; aging

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type

NCBI Summary:This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
UniProt Code:P02533
NCBI GenInfo Identifier:229463044
NCBI Gene ID:3861
NCBI Accession:P02533.4
UniProt Secondary Accession:P02533,P08779,
UniProt Related Accession:P02533
Molecular Weight:~ 52kDa
NCBI Full Name:Keratin, type I cytoskeletal 14
NCBI Synonym Full Names:keratin 14
NCBI Official Symbol:KRT14��
NCBI Official Synonym Symbols:K14; NFJ; CK14; EBS3; EBS4��
NCBI Protein Information:keratin, type I cytoskeletal 14
UniProt Protein Name:Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names:Cytokeratin-14; CK-14; Keratin-14; K14
Protein Family:Putative outer capsid protein
UniProt Gene Name:KRT14��
UniProt Entry Name:K1C14_HUMAN
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